An acute intake of plant stanol esters alters immune-related pathways in the jejunum of healthy volunteers
| Autor: | Mark V. Boekschoten, Jogchum Plat, Ronald P. Mensink, Tim G. A. M. Wolfs, Wilfred T. V. Germeraad, Rogier de Ridder, Els De Smet |
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| Přispěvatelé: | Promovendi NTM, Humane Biologie, MUMC+: MA Maag Darm Lever (9), Interne Geneeskunde, MUMC+: MA Hematologie (9), Kindergeneeskunde, RS: GROW - Oncology, RS: NUTRIM - R1 - Metabolic Syndrome, RS: NUTRIM - R2 - Gut-liver homeostasis, RS: GROW - R3 - Innovative Cancer Diagnostics & Therapy, RS: GROW - R4 - Reproductive and Perinatal Medicine |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
cholesterol-metabolism T-Lymphocytes Medicine (miscellaneous) Intestinal gene expression profiles Jejunum Voeding Metabolisme en Genomica sterol-metabolism Intestinal mucosa abcg8 Intestinal Mucosa triglyceride transfer protein Cross-Over Studies Nutrition and Dietetics atp-binding cassette Anticholesteremic Agents Forkhead Transcription Factors Middle Aged Metabolism and Genomics medicine.anatomical_structure messenger-rna Metabolisme en Genomica Antigens Surface Intestinal cholesterol absorption Female Nutrition Metabolism and Genomics Adult medicine.medical_specialty Duodenum Down-Regulation ABCG8 Immune function Biology Beverages Immunomodulation Young Adult Double-Blind Method Voeding Internal medicine expression medicine Humans beta-sitosterol Immunity Mucosal Nutrition VLAG Global Nutrition Wereldvoeding Microarray analysis dietary phytosterols Sitosterols Small intestine niemann-pick c1-like-1 Major duodenal papilla Endocrinology Gene Expression Regulation Plant stanol ester Plant stanol esters |
| Zdroj: | British Journal of Nutrition, 113(5), 794-802. Cambridge University Press British Journal of Nutrition 113 (2015) 5 British Journal of Nutrition, 113(5), 794-802 |
| ISSN: | 0007-1145 |
| DOI: | 10.1017/s000711451400350x |
| Popis: | Plant sterols and stanols inhibit intestinal cholesterol absorption and consequently lower serum LDL-cholesterol (LDL-C) concentrations. The underlying mechanisms are not yet known.In vitroand animal studies have suggested that changes in intestinal sterol metabolism are attributed to the LDL-C-lowering effects of plant stanol esters. However, similar studies in human subjects are lacking. Therefore, we examined the effects of an acute intake of plant stanol esters on gene expression profiles of the upper small intestine in healthy volunteers. In a double-blind cross-over design, fourteen healthy subjects (eight female and six male; age 21–55 years), with a BMI ranging from 21 to 29 kg/m2, received in random order a shake with or without plant stanol esters (4 g). At 5 h after consumption of the shake, biopsies were taken from the duodenum (around the papilla of Vater) and from the jejunum (20 cm distal from the papilla of Vater). Microarray analysis showed that the expression profiles of genes involved in sterol metabolism were not altered. Surprisingly, the pathways involved in T-cell functions were down-regulated in the jejunum. Furthermore, immunohistochemical analysis showed that the number of CD3 (cluster of differentiation number 3), CD4 (cluster of differentiation number 4) and Foxp3+(forkhead box P3-positive) cells was reduced in the plant stanol ester condition compared with the control condition, which is in line with the microarray data. The physiological and functional consequences of the plant stanol ester-induced reduction of intestinal T-cell-based immune activity in healthy subjects deserve further investigation. |
| Databáze: | OpenAIRE |
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