Exponential increase in neutralizing and spike specific antibodies following vaccination of COVID ‐19 convalescent plasma donors
| Autor: | Anna E. Merrill, Abby E. Odle, Myrl Holida, Kerry A. Dubay, C. Michael Knudson, Judith Leon, Aaron V. Locher, Molly A. Vickers, Laura Collins, Stanley Perlman, Alexandra Ehlers, Alan Sariol, Dena R. Voss |
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| Rok vydání: | 2021 |
| Předmět: |
Male
COVID-19 Vaccines Convalescent plasma Coronavirus disease 2019 (COVID-19) Immunology Blood Donors Enzyme-Linked Immunosorbent Assay immunology (other than RBC serology) 030204 cardiovascular system & hematology Antibodies Viral Neutralization 03 medical and health sciences 0302 clinical medicine Humans Immunology and Allergy Medicine Neutralizing antibody Original Research biology SARS-CoV-2 business.industry Immune Sera Vaccination COVID-19 Hematology Middle Aged Antibodies Neutralizing Specific antibody Titer Immunoglobulin G Spike Glycoprotein Coronavirus biology.protein Female transfusion Practices (Adult) Antibody business 030215 immunology |
| Zdroj: | Transfusion |
| ISSN: | 1537-2995 0041-1132 |
| Popis: | Background With the recent approval of COVID‐19 vaccines, recovered COVID‐19 subjects who are vaccinated may be ideal candidates to donate COVID‐19 convalescent plasma (CCP). Case Series Eleven recovered COVID‐19 patients were screened to donate CCP. All had molecularly confirmed COVID‐19, and all but one were antibody positive by chemiluminescence immunoassay (DiaSorin) prior to vaccination. All were tested again for antibodies 11–21 days after they were vaccinated (Pfizer/Moderna). All showed dramatic increases (~50‐fold) in spike‐specific antibody levels and had at least a 20‐fold increase in the IC50 neutralizing antibody titer based on plaque reduction neutralization testing (PRNT). The spike‐specific antibody levels following vaccination were significantly higher than those seen in any non‐vaccinated COVID‐19 subjects tested to date at our facility. Conclusion Spike‐specific and neutralizing antibodies demonstrated dramatic increases following a single vaccination after COVID‐19 infection, which significantly exceeded values seen with COVID‐19 infection alone. Recovered COVID‐19 subjects who are vaccinated may make ideal candidates for CCP donation. |
| Databáze: | OpenAIRE |
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