The quantitative estimation of pinocytosis using radioactive colloidal gold
Autor: | Philip Davies, A. David Haswell, Anthony C. Allison |
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Rok vydání: | 1973 |
Předmět: | |
Zdroj: | Biochemical and biophysical research communications. 52(2) |
ISSN: | 0006-291X |
Popis: | Radioactive colloidal gold has been used as a quantitative indicator of the pinocytic activity of mouse peritoneal macrophages. The uptake of label is both time and temperature dependent, accumulation being linearly related to time for several hours. Uptake is also dependent on the number of cells and concentration of the radioactive label. Increasing concentrations of newborn calf serum in the culture medium cause dramatic increases in pinocytosis without corresponding increases in protein levels in the cells. |
Databáze: | OpenAIRE |
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