Mast cell tryptase enhances wound healing by promoting migration in human bronchial epithelial cells
| Autor: | Frida Berlin, Sangeetha Ramu, Celeste Porsbjerg, Asger Sverrild, Sofia Mogren, Lena Uller, Cecilia Andersson |
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| Rok vydání: | 2021 |
| Předmět: |
Cell
tryptase protease activated receptor 2 Bronchi Tryptase Context (language use) Mast cell Cell Line Epithelial Damage Cellular and Molecular Neuroscience Cell Movement medicine Humans Receptor PAR-2 Proliferation Marker Mast Cells Protease-activated receptor 2 Wound Healing QH573-671 biology Chemistry Epithelial Cells Cell Biology medicine.anatomical_structure biology.protein Cancer research Tryptases Cytology Wound healing Research Article Research Paper |
| Zdroj: | Mogren, S, Berlin, F, Ramu, S, Sverrild, A, Porsbjerg, C, Uller, L & Andersson, C K 2021, ' Mast cell tryptase enhances wound healing by promoting migration in human bronchial epithelial cells ', Cell Adhesion and Migration, vol. 15, no. 1, pp. 202-214 . https://doi.org/10.1080/19336918.2021.1950594 Cell Adhesion & Migration article-version (VoR) Version of Record Cell Adhesion & Migration, Vol 15, Iss 1, Pp 202-214 (2021) |
| ISSN: | 1933-6926 1933-6918 |
| DOI: | 10.1080/19336918.2021.1950594 |
| Popis: | Epithelial damage and increase of intraepithelial mast cells (MC) are characteristics of asthma. The role of MC mediator tryptase and the protease-activated receptor-2 (PAR2) on epithelial wound healing is not fully investigated. Stimulation of bronchial epithelial cells (BECs) with tryptase promoted gap closure, migration and cellular speed compared to controls. Stimulated BECs had higher expression of migration marker CD151 compared to controls. Proliferation marker KI67 was upregulated in tryptase-stimulated BECs compared to controls. Treatment with PAR2 antagonist I-191 reduced gap closure, migration and cell speed compared to BECs stimulated with tryptase. We found that tryptase enhances epithelial wound healing by increased migration and proliferation, which is in part regulated via PAR2. Our data suggest that tryptase might be beneficial in tissue repair under baseline conditions. However, in a pathological context such as asthma with increased numbers of activated MCs, it might lead to epithelial remodeling and loss of function. |
| Databáze: | OpenAIRE |
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