Self-Assembled Nanoparticles Based on Block-Copolymers of Poly(2-Deoxy-2-methacrylamido-d-glucose)/Poly(N-Vinyl Succinamic Acid) with Poly(O-Cholesteryl Methacrylate) for Delivery of Hydrophobic Drugs
| Autor: | Alena Vdovchenko, Evgenia Korzhikova-Vlakh, Apollinariia Dzhuzha, Eugene Sivtsov, Alexey Gostev, M. L. Levit, Natalia Zashikhina, Antonina Lavrentieva, Elena Katernyuk, Tatiana B. Tennikova |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Paclitaxel
block-copolymers Polymers QH301-705.5 paclitaxel delivery Dispersity Nanoparticle bio-inspired copolymers Methacrylate Article Catalysis Cell Line Inorganic Chemistry chemistry.chemical_compound drug delivery systems Polymer chemistry Amphiphile Copolymer Humans Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy chemistry.chemical_classification Organic Chemistry General Medicine Polymer Computer Science Applications Chemistry HEK293 Cells Monomer Polymerization chemistry controlled radical polymerization polymer nanoparticles Methacrylates Nanoparticles amphiphilic copolymers Hydrophobic and Hydrophilic Interactions |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 21 International Journal of Molecular Sciences, Vol 22, Iss 11457, p 11457 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms222111457 |
| Popis: | The self-assembly of amphiphilic block-copolymers is a convenient way to obtain soft nanomaterials of different morphology and scale. In turn, the use of a biomimetic approach makes it possible to synthesize polymers with fragments similar to natural macromolecules but more resistant to biodegradation. In this study, we synthesized the novel bio-inspired amphiphilic block-copolymers consisting of poly(N-methacrylamido-d-glucose) or poly(N-vinyl succinamic acid) as a hydrophilic fragment and poly(O-cholesteryl methacrylate) as a hydrophobic fragment. Block-copolymers were synthesized by radical addition–fragmentation chain-transfer (RAFT) polymerization using dithiobenzoate or trithiocarbonate chain-transfer agent depending on the first monomer, further forming the hydrophilic block. Both homopolymers and copolymers were characterized by 1H NMR and Fourier transform infrared spectroscopy, as well as thermogravimetric analysis. The obtained copolymers had low dispersity (1.05–1.37) and molecular weights in the range of ~13,000–32,000. The amphiphilic copolymers demonstrated enhanced thermal stability in comparison with hydrophilic precursors. According to dynamic light scattering and nanoparticle tracking analysis, the obtained amphiphilic copolymers were able to self-assemble in aqueous media into nanoparticles with a hydrodynamic diameter of approximately 200 nm. An investigation of nanoparticles by transmission electron microscopy revealed their spherical shape. The obtained nanoparticles did not demonstrate cytotoxicity against human embryonic kidney (HEK293) and bronchial epithelial (BEAS-2B) cells, and they were characterized by a low uptake by macrophages in vitro. Paclitaxel loaded into the developed polymer nanoparticles retained biological activity against lung adenocarcinoma epithelial cells (A549). |
| Databáze: | OpenAIRE |
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