Development and characterization of recombinant tick-borne encephalitis virus expressing mCherry reporter protein: A new tool for high-throughput screening of antiviral compounds, and neutralizing antibody assays
| Autor: | Jiri Volf, Jean-Sélim Driouich, Daniel Ruzek, Kentaro Yoshii, Jiri Cerny, Ludek Eyer, Martin Palus, Antoine Nougairède, Xavier de Lamballerie, Ernest A. Gould, Shintaro Kobayashi, Jan Haviernik |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
030106 microbiology Antibodies Viral Kidney Antiviral Agents Virus Cell Line Encephalitis Viruses Tick-Borne law.invention 03 medical and health sciences Neutralization Tests law Cricetinae Virology medicine Animals Humans Neutralizing antibody Pharmacology biology medicine.disease biology.organism_classification Antibodies Neutralizing High-Throughput Screening Assays 3. Good health Luminescent Proteins Flavivirus Tick-borne encephalitis virus 030104 developmental biology biology.protein Recombinant DNA Antibody mCherry Encephalitis Tick-Borne Encephalitis |
| Zdroj: | Antiviral Research. 185:104968 |
| ISSN: | 0166-3542 |
| Popis: | The flavivirus, tick-borne encephalitis virus (TBEV) is transmitted by Ixodes spp. ticks and may cause severe and potentially lethal neurological tick-borne encephalitis (TBE) in humans. Studying TBEV requires the use of secondary methodologies to detect the virus in infected cells. To overcome this problem, we rationally designed and constructed a recombinant reporter TBEV that stably expressed the mCherry reporter protein. The resulting TBEV reporter virus (named mCherry-TBEV) and wild-type parental TBEV exhibited similar growth kinetics in cultured cells; however, the mCherry-TBEV virus produced smaller plaques. The magnitude of mCherry expression correlated well with progeny virus production but remained stable over |
| Databáze: | OpenAIRE |
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