Evaluation of chromosomal abnormalities by cIg-FISH and association with proliferative and apoptotic indexes in multiple myeloma
Autor: | L. M. Pelicario, Elvira Deolinda Rodrigues Pereira Velloso, Valeria Buccheri, AM Leal, Pedro Enrique Dorlhiac-Llacer, Cristina Aiko Kumeda, Camila da Cruz Gouveia Linardi, Gracia Martinez, Raymundo Soares Azevedo |
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Rok vydání: | 2012 |
Předmět: |
Adult
Male Pathology medicine.medical_specialty Physiology Short Communication Plasma Cells Immunology Biophysics Apoptosis Biology Plasma cell Biochemistry Gastroenterology Flow cytometry Bone Marrow Multiple myeloma hemic and lymphatic diseases Internal medicine medicine Humans General Pharmacology Toxicology and Pharmaceutics lcsh:QH301-705.5 In Situ Hybridization Fluorescence Cell proliferation Survival analysis Aged Aged 80 and over Chromosome Aberrations lcsh:R5-920 medicine.diagnostic_test Fluorescence in situ hybridization General Neuroscience Cell Biology General Medicine Middle Aged Flow Cytometry Prognosis medicine.disease Survival Analysis Pathophysiology medicine.anatomical_structure lcsh:Biology (General) Female Bone marrow Abnormality lcsh:Medicine (General) |
Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 45, Iss 11, Pp 1074-1079 (2012) Brazilian Journal of Medical and Biological Research, Volume: 45, Issue: 11, Pages: 1074-1079, Published: NOV 2012 Brazilian Journal of Medical and Biological Research |
ISSN: | 0100-879X |
Popis: | Eighty-six newly diagnosed multiple myeloma (MM) patients from a public hospital of São Paulo (Brazil) were evaluated by cIg-FISH for the presence of del(13)(q14), t(4;14)(p16.3;q32) and del(17)(p13). These abnormalities were observed in 46.5, 9.3, and 7.0% of the patients, respectively. In order to identify the possible role of del(13)(q14) in the physiopathology of MM, we investigated the association between this abnormality and the proliferative and apoptotic indexes of plasma cells. When cases demonstrating t(4;14)(p16.3;q32) and del(17)(p13) were excluded from the analysis, we observed a trend towards a positive correlation between the proportion of cells carrying del(13)(q14) and plasma cell proliferation, determined by Ki-67 expression (r = 0.23, P = 0.06). On the other hand, no correlation between the proportion of cells carrying del(13)(q14) and apoptosis, determined by annexin-V staining, was detected (r = 0.05, P = 0.69). In general, patients carrying del(13)(q14) did not have lower survival than patients without del(13)(q14) (P = 0.15), but patients with more than 80% of cells carrying del(13)(q14) showed a lower overall survival (P = 0.033). These results suggest that, when del(13)(q14) is observed in a high proportion of malignant cells, it may have a role in determining MM prognosis. Another finding was a statistically significant lower overall survival of patients with t(4;14)(p16.3;q32) (P = 0.026). In the present study, almost half the patients with t(4;14)(p16.3;q32) died just after diagnosis, before starting treatment. This fact suggests that, in São Paulo, there may be even more patients with this chromosomal abnormality, but they probably die before being diagnosed due to unfavorable socioeconomic conditions. This could explain the low prevalence of this chromosomal abnormality observed in the present study. |
Databáze: | OpenAIRE |
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