Isolation and Characterization of Mouse Minisatellites
| Autor: | Sharmila Bakshi, Alec J. Jeffreys, Philippe Bois, Behzad Moghadaszadeh, John D.H. Stead, Rita Neumann, Jill Williamson |
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| Rok vydání: | 1998 |
| Předmět: |
Genetic Linkage
Minisatellite Repeat Molecular Sequence Data Mice Inbred Strains Minisatellite Repeats Biology Polymerase Chain Reaction Genome Mice Gene mapping Tandem repeat Genetics Animals Cloning Molecular Alleles Germ-Line Mutation In Situ Hybridization Fluorescence Genomic organization Genomic Library Polymorphism Genetic Base Sequence Chromosome Mapping Sequence Analysis DNA Cosmids Molecular Weight Variable number tandem repeat Minisatellite Genetic marker Female |
| Zdroj: | Genomics. 50:317-330 |
| ISSN: | 0888-7543 |
| Popis: | Minisatellites provide the most informative system for analyzing processes of tandem repeat turnover in humans. However, little is known about minisatellites and the mechanisms by which they mutate in other species. To this end, we have isolated and characterized 76 endogenous mouse VNTRs. Fifty-one loci have been localized on mouse chromosomes and, unlike in humans, show no clustering in proterminal regions. Sequence analysis of 25 loci revealed the majority to be authentic minisatellites with GC-rich repeat units ranging from 14 to 47 bp in length. We have further characterized 3 of the most polymorphic loci both in Mus musculus subspecies and in inbred strains by using minisatellite variant repeat mapping (MVR) by PCR to gain insight into allelic diversity and turnover processes. MVR data suggest that mouse minisatellites mutate mainly by intra-allelic nonpolar events at a rate well below 10(-3) per gamete, in contrast to the high-frequency complex meiotic gene conversion-like events seen in humans. These results may indicate a fundamental difference in mechanisms of minisatellite mutation and genome turnover between mice and humans. |
| Databáze: | OpenAIRE |
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