Protective role of soluble adenylyl cyclase against reperfusion-induced injury of cardiac cells
| Autor: | Vignesh Jayarajan, Laura Rinaldi, Yury Ladilov, Sofya Pozdniakova, Yaser Abdallah, Muhammad Aslam, Christian Troidl |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Programmed cell death Cell Survival Mitochondria Heart Cell Line Necrosis 03 medical and health sciences Cytosol 0302 clinical medicine Cyclic AMP medicine Animals Myocytes Cardiac Viability assay Rats Wistar education Molecular Biology education.field_of_study Activator (genetics) Chemistry Hydrogen-Ion Concentration Soluble adenylyl cyclase medicine.disease Cyclic Nucleotide Phosphodiesterases Type 2 Rats Cell biology Disease Models Animal 030104 developmental biology Reperfusion Injury Molecular Medicine Phosphodiesterase 2 Reperfusion injury 030217 neurology & neurosurgery Homeostasis Adenylyl Cyclases Signal Transduction |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1865:252-260 |
| ISSN: | 0925-4439 |
| Popis: | Aims Disturbance of mitochondrial function significantly contributes to the myocardial injury that occurs during reperfusion. Increasing evidence suggests a role of intra-mitochondrial cyclic AMP (cAMP) signaling in promoting respiration and ATP synthesis. Mitochondrial levels of cAMP are controlled by type 10 soluble adenylyl cyclase (sAC) and phosphodiesterase 2 (PDE2), however their role in the reperfusion-induced injury remains unknown. Here we aimed to examine whether sAC may support cardiomyocyte survival during reperfusion. Methods and results Adult rat cardiomyocytes or rat cardiac H9C2 cells were subjected to metabolic inhibition and recovery as a model of simulated ischemia and reperfusion. Cytosolic Ca2+, pH, mitochondrial cAMP (live-cell imaging), and cell viability were analyzed during a 15-min period of reperfusion. Suppression of sAC activity in cardiomyocytes and H9C2 cells, either by sAC knockdown, by pharmacological inhibition or by withdrawal of bicarbonate, a natural sAC activator, compromised cell viability and recovery of cytosolic Ca2+ homeostasis during reperfusion. Contrariwise, overexpression of mitochondria-targeted sAC in H9C2 cells suppressed reperfusion-induced cell death. Analyzing cAMP concentration in mitochondrial matrix we found that inhibition of PDE2, a predominant mitochondria-localized PDE isoform in mammals, during reperfusion significantly increased cAMP level in mitochondrial matrix, but not in cytosol. Accordingly, PDE2 inhibition attenuated reperfusion-induced cardiomyocyte death and improved recovery of the cytosolic Ca2+ homeostasis. Conclusion sAC plays an essential role in supporting cardiomyocytes viability during reperfusion. Elevation of mitochondrial cAMP pool either by sAC overexpression or by PDE2 inhibition beneficially affects cardiomyocyte survival during reperfusion. |
| Databáze: | OpenAIRE |
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