Detection of Enterobacterial Lipopolysaccharides and Experimental Endotoxemia by Means of an Immunolimulus Assay Using Both SerotypeSpecific and Cross-Reactive Antibodies
| Autor: | A. Lindberg, S. J. Cryz, Harri Saxen, F. di Padova, Jaana Vuopio-Varkila, Jussi Mertsola, Eric J. Hansen, Alois B. Lang, J. Luk, George H. McCracken |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Lipopolysaccharides
Salmonella Lipopolysaccharide medicine.drug_class Toxemia Cross Reactions medicine.disease_cause Monoclonal antibody Sensitivity and Specificity Microbiology Rats Sprague-Dawley chemistry.chemical_compound Mice Antigen Enterobacteriaceae medicine Immunology and Allergy Animals Humans Shigella Serotyping Escherichia coli Escherichia coli Infections Limulus Test biology Infant Newborn Antibodies Monoclonal biology.organism_classification Antibodies Bacterial Rats Endotoxins Mice Inbred C57BL Infectious Diseases chemistry Animals Newborn Limulus amebocyte lysate Mice Inbred CBA |
| Zdroj: | Saxen, Harri; Vuopio-Varkila, Jaana; Luk, John; Lindberg, Alf; Lang, Alois; Di Padova, Franco; Cryz, Stanley J.; Mertsola, Jussi; Mccracken, George H.; Hansen, Eric J. (1993). Detection of Enterobacterial Lipopolysaccharides and Experimental Endotoxemia by Means of an Immunolimulus Assay Using Both Serotype-Specific and Cross-Reactive Antibodies. Journal of infectious diseases, 168(2), pp. 393-399. The University of Chicago Press 10.1093/infdis/168.2.393 Scopus-Elsevier |
| DOI: | 10.1093/infdis/168.2.393 |
| Popis: | The immunolimulus (IML) assay system uses solid-phase endotoxin antibodies to capture lipopolysaccharide (LPS), which is then quantified by a modification of the chromogenic limulus amebocyte lysate (CLAL) method. Monoclonal antibodies (MAbs) reactive with selected 0 antigen serotypes of Escherichia coli (O18) and Salmonella typhimurium (O-9,12), when used in the IML, were shown to be highly specific in detecting their respective endotoxins in purified form and in plasma samples from experimentally infected animals. A murine MAb that was broadly cross-reactive with E. coli, Salmonella, and Shigella endotoxins also proved to be highly effective in the IML assay for capturing LPS molecules from both E. coli and S. typhimurium strains. These results indicate that IML assays can detect smooth-type enterobacterial endotoxins in plasma and suggest that such assays have potential for use in the rapid diagnosis of sepsis and endotoxemia caused by different enterobacterial species. |
| Databáze: | OpenAIRE |
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