Assessment of mutations induced by cold atmospheric plasma jet treatment relative to known mutagens in Escherichia coli
| Autor: | Hollie Hathaway, Robert D. Short, A. Toby A. Jenkins, Naing Tun Thet, Bethany L. Patenall, Maisem Laabei, Amber Young, Sarah L. Allinson |
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| Rok vydání: | 2021 |
| Předmět: |
Plasma Gases
Ultraviolet Rays DNA damage medicine.drug_class Health Toxicology and Mutagenesis Mutant Antibiotics DNA Fragmentation Toxicology medicine.disease_cause Ames test Drug Resistance Bacterial Escherichia coli Genetics medicine Genetics (clinical) Mutation Dose-Response Relationship Drug Chemistry X-Rays Mutagenesis Molecular biology DNA fragmentation DNA Damage Mutagens |
| Zdroj: | Mutagenesis. 36:380-387 |
| ISSN: | 1464-3804 0267-8357 |
| Popis: | The main bactericidal components of cold atmospheric plasma (CAP) are thought to be reactive oxygen and nitrogen species (RONS) and UV-radiation, both of which have the capacity to cause DNA damage and mutations. Here, the mutagenic effects of CAP on Escherichia coli were assessed in comparison to X- and UV-irradiation. DNA damage and mutagenesis were screened for using a diffusion-based DNA fragmentation assay and modified Ames test, respectively. Mutant colonies obtained from the latter were quantitated and sequenced. CAP was found to elicit a similar mutation spectrum to X-irradiation, which did not resemble that for UV implying that CAP-produced RONS are more likely the mutagenic component of CAP. CAP treatment was also shown to promote resistance to the antibiotic ciprofloxacin. Our data suggest that CAP treatment has mutagenic effects that may have important phenotypic consequences. |
| Databáze: | OpenAIRE |
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