REG1 binds to protein phosphatase type 1 and regulates glucose repression in Saccharomyces cerevisiae
| Autor: | Marian Carlson, Jiangling Tu |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Protein subunit Recombinant Fusion Proteins Phosphatase Saccharomyces cerevisiae Immunoblotting Molecular Sequence Data macromolecular substances General Biochemistry Genetics and Molecular Biology Fungal Proteins chemistry.chemical_compound Gene Expression Regulation Fungal Protein Phosphatase 1 Phosphoprotein Phosphatases Molecular Biology Fungal protein General Immunology and Microbiology Glycogen biology Base Sequence General Neuroscience fungi Protein phosphatase 1 Protein phosphatase 2 biology.organism_classification Fusion protein Precipitin Tests Glucose Biochemistry chemistry Mutation Research Article |
| Zdroj: | The EMBO journal. 14(23) |
| ISSN: | 0261-4189 |
| Popis: | Protein phosphatase type 1 (PP1) is encoded by GLC7, an essential gene in Saccharomyces cerevisiae. The GLC7 phosphatase is required for glucose repression and appears to function antagonistically to the SNF1 protein kinase. Previously, we characterized a mutation, glc7-T152K, that relieves glucose repression but does not interfere with the function of GLC7 in glycogen metabolism. We proposed that the mutant GLC7T152K phosphatase is defective in its interaction with a regulatory subunit that directs participation of PP1 in the glucose repression mechanism. Here, we present evidence that REG1, a protein required for glucose repression, is one such regulatory subunit. We show that REG1 is physically associated with GLC7. REG1 interacts with GLC7 strongly and specifically in the two-hybrid system, and REG1 and GLC7 fusion proteins co-immunoprecipitate from cell extracts. Moreover, overexpression of a REG1 fusion protein suppresses the glc7-T152K mutant defect in glucose repression. This and other genetic evidence indicate that the two proteins function together in regulating glucose repression. These results suggest that REG1 is a regulatory subunit of PP1 that targets its activity to proteins in the glucose repression regulatory pathway. |
| Databáze: | OpenAIRE |
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