Selective chemical inhibition as a tool to study Cdk1 and Cdk2 functions in the cell cycle
| Autor: | Daniel Fisher, Liliana Krasinska, Emilie Cot |
|---|---|
| Přispěvatelé: | Institut de Génétique Moléculaire de Montpellier ( IGMM ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Phosphorylation and control of the vertebrate cell cycle, Institut National de la Santé et de la Recherche Médicale ( INSERM ), Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Institut National de la Santé et de la Recherche Médicale (INSERM), Fisher, Daniel, Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Xenopus
MESH: Cell Cycle MESH: DNA Replication chemical inhibitors 0302 clinical medicine MESH: Ovum MESH : Thiazoles Nu6102 MESH : Ovum MESH: Protein Kinase Inhibitors MESH: Animals MESH: Xenopus MESH : Purines Cells Cultured 0303 health sciences biology Kinase Cell Cycle MESH : Cyclin-Dependent Kinase 2 MESH: Purines Cell cycle MESH: CDC2 Protein Kinase Chromatin Cell biology 030220 oncology & carcinogenesis Cyclin dependent kinase Quinolines biological phenomena cell phenomena and immunity MESH: Quinolines MESH: Cells Cultured MESH : DNA Replication DNA Replication MESH: Cyclin-Dependent Kinase 2 MESH : CDC2 Protein Kinase MESH : Quinolines MESH: Thiazoles Article 03 medical and health sciences Cyclin-dependent kinase fibroblasts CDC2 Protein Kinase MESH : Cell Cycle MESH : Cells Cultured [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Animals Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Protein Kinase Inhibitors Molecular Biology Mitosis [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biology Gene knockout Ovum 030304 developmental biology Cyclin-dependent kinase 1 MESH: Humans Cyclin-Dependent Kinase 2 MESH : Humans MESH : Xenopus Cell Biology biology.organism_classification MESH : Protein Kinase Inhibitors Thiazoles Purines biology.protein chromatin MESH : Animals Developmental Biology RO-3306 |
| Zdroj: | Cell Cycle Cell Cycle, Taylor & Francis, 2008, 7 (12), pp.1702-8 Cell Cycle, Taylor & Francis, 2008, 7 (12), pp.1702-8. ⟨10.4161/cc.7.12.6101⟩ Cell Cycle, 2008, 7 (12), pp.1702-8. ⟨10.4161/cc.7.12.6101⟩ |
| ISSN: | 1538-4101 1551-4005 |
| DOI: | 10.4161/cc.7.12.6101⟩ |
| Popis: | International audience; Cyclin-dependent kinases are highly conserved among all eukaryotes, and have essential roles in the cell cycle. However, these roles are still only poorly understood at a molecular level, partly due to the functional redundancy of different Cdk complexes. Indeed, mice knockouts have even thrown into some doubt the assumed essential roles for Cdk2-cyclin E in triggering S-phase, but this is almost certainly due to compensation by Cdk1 complexes. By combining both knockout approaches and chemical Cdk inhibition in Xenopus egg extracts, we have shown that one reason for functional redundancy of Cdk control of S-phase is that Cdk activity required to trigger S-phase is very low. Cdk1 contributes to this activity even in the presence of Cdk2, and Cdk activity at this stage does not show "switch-like" regulation, as at the onset of mitosis. It is important to try to confirm and extend these findings to other cell-types, and to explain why different cells might have evolved different requirements for Cdk activity. In this paper, we present data that suggest that selective chemical Cdk inhibition will be a useful tool towards achieving this goal. |
| Databáze: | OpenAIRE |
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