Multimodal CARS microscopy of structured carbohydrate biopolymers

Autor: Albert Stolow, Aaron D. Slepkov, Adrian F. Pegoraro, Andrew Ridsdale, Douglas J. Moffatt
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Zdroj: Biomedical Optics Express
ISSN: 2156-7085
Popis: We demonstrate the utility of multimodal coherent anti-Stokes Raman scattering (CARS) microscopy for the study of structured condensed carbohydrate systems. Simultaneous second-harmonic generation (SHG) and spectrally-scanned CARS microscopy was used to elucidate structure, alignment, and density in cellulose cotton fibers and in starch grains undergoing rapid heat-moisture swelling. Our results suggest that CARS response of the O-H stretch region (3000 cm(-1)-3400 cm(-1)), together with the commonly-measured C-H stretch (2750 cm(-1)-2970 cm(-1)) and SHG provide potentially important structural information and contrast in these materials.
Databáze: OpenAIRE