Direct expression of urogastrone gene in Escherichia coli
| Autor: | Nakatani T, Fumitaka Kishimoto, Masaharu Kanaoka, Hideo Agui, Akira Ito, Shigeo Ogino, Katoh T, Sumida S, Hideyuki Gomi |
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| Rok vydání: | 1986 |
| Předmět: |
Biology
medicine.disease_cause law.invention chemistry.chemical_compound Plasmid Epidermal growth factor law Escherichia coli Genetics medicine Humans Coding region Amino Acid Sequence Cloning Molecular Gene Base Sequence Epidermal Growth Factor DNA Restriction Enzymes General Medicine Molecular biology Genes chemistry Genes Bacterial Recombinant DNA Bacteriophage Mu DNA Bacterial Outer Membrane Proteins Plasmids |
| Zdroj: | Gene. 45:311-316 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(86)90029-6 |
| Popis: | Human epidermal growth factor (urogastrone; UG) is a 53-amino acid polypeptide hormone. A 192-bp DNA fragment containing the coding sequence for methionyl UG (Met-UG) and the ribosome-binding site (RBS) was chemically synthesized and placed downstream from the promoter for the Escherichia coli outer-membrane lipoprotein gene (Ipp) on a plasmid. E. coli cells harboring the plasmid directed the synthesis of Met-UG at 10 2 -10 2 molecules per cell. Next, the coding sequence for Met-UG was inserted in a runaway-replication plasmid and expressed under the control of the lpp promoter and the RBS derived from bacteriophage Mu cII gene. Upon heat induction, the cells harboring the recombinant plasmid synthesized 10 5 molecules of Met-UG per cell. |
| Databáze: | OpenAIRE |
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