Biopsy mineral analysis by inductively coupled plasma-atomic emission spectroscopy with ultrasonic nebulization
| Autor: | Kirk J. Stuart, Thomas H. Herdt, Thomas P. Mullaney, W. Emmett Braselton |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Materials science Vena cava 040301 veterinary sciences Biopsy 030106 microbiology chemistry.chemical_element Nutritional Status 0403 veterinary science 03 medical and health sciences medicine Animals Ultrasonics Liver copper Chromatography General Veterinary medicine.diagnostic_test Mineral analysis Nebulizers and Vaporizers Spectrophotometry Atomic 04 agricultural and veterinary sciences Copper Ultrasonic nebulization Trace Elements chemistry Liver Organ Specificity Inductively coupled plasma atomic emission spectroscopy Needle biopsy Cattle |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 9(4) |
| ISSN: | 1040-6387 |
| Popis: | Liver copper concentration is generally considered the best measure of copper nutritional status in cattle. Ultrasonic nebulization in conjunction with inductively coupled plasma-atomic emission spectroscopy (ICP—AES) was investigated as a method to provide adequate sensitivity to allow accurate simultaneous measurement of copper and 14 additional elements from needle biopsy samples. The element concentration frequency distribution profile of 12 elements routinely present in liver was compared to profiles of the elements in fat, muscle, vena cava, kidney, and clotted blood. The profiles could be used to confirm the authenticity of the liver biopsy sample. Element concentrations in biopsy samples taken in triplicate from the five lobes of a bovine liver were compared to those from triplicate wedge sections taken adjacent to the biopsies and analyzed by conventional ICP—AES. Precision between biopsies was equal to or better than precision between wedge samples. Some element concentrations determined by the biopsy procedure differed statistically from those determined by the wedge procedure, but differences were not sufficient to influence clinical interpretation of data. |
| Databáze: | OpenAIRE |
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