Changes in steady-state concentrations of messenger ribonucleic acids in luteal tissue during prostaglandin F2α induced luteolysis in mares
| Autor: | Mohd A. Beg, M. O. Gastal, Milo C. Wiltbank, S. Ji, O.J. Ginther, Eduardo L. Gastal |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Ovulation
endocrine system medicine.medical_specialty Time Factors Biopsy media_common.quotation_subject medicine.medical_treatment Luteolysis Prostaglandin Luteal phase Biology Dinoprost chemistry.chemical_compound Aromatase Endocrinology Food Animals Corpus Luteum Internal medicine medicine Animals Horses RNA Messenger Saline Progesterone media_common medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Steroidogenic acute regulatory protein General Medicine Receptors LH Phosphoproteins medicine.anatomical_structure chemistry Cyclooxygenase 2 Female lipids (amino acids peptides and proteins) Animal Science and Zoology Corpus luteum |
| Zdroj: | Animal Reproduction Science. 90:273-285 |
| ISSN: | 0378-4320 |
| DOI: | 10.1016/j.anireprosci.2005.02.008 |
| Popis: | Transvaginal ultrasound-guided luteal biopsy was used to evaluate the effects of prostaglandin (PG)F2alpha on steady-state concentrations of mRNA for specific genes that may be involved in regression of the corpus luteum (CL). Eight days after ovulation (Hour 0), mares (n=8/group) were randomized into three groups: control (no treatment or biopsy), saline+biopsy (saline treatment at Hour 0 and luteal biopsy at Hour 12), or PGF2alpha+biopsy (5mg PGF2alpha at Hour 0 and luteal biopsy at Hour 12). The effects of biopsy on CL were compared between the controls (no biopsy) and saline+biopsy group. At Hour 24 (12h after biopsy) there was a decrease in circulating progesterone in saline group to 56% of pre-biopsy values, indicating an effect of biopsy on luteal function. Mean plasma progesterone concentrations were lower (P |
| Databáze: | OpenAIRE |
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