Distinguished effects of antiphospholipid antibodies and anti-oxidized LDL antibodies on oxidized LDL uptake by macrophages
| Autor: | Tatsuji Yasuda, Masataka Kuwana, H. Itabe, T. Ueno, Akito Tsutsumi, Eiji Matsuura, Kazuko Kobayashi, K. Tada, P. H. Liu, P. G. De Groot, Y Shoenfeld |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Apolipoprotein E
medicine.drug_class Monoclonal antibody Cell Line Mice chemistry.chemical_compound Rheumatology Antibody Specificity Animals Humans Medicine Beta 2-Glycoprotein I biology business.industry Macrophages Autoantibody Immunoglobulins Intravenous Antiphospholipid Syndrome Atherosclerosis Molecular biology Lipoproteins LDL Blot Immunoglobulin M chemistry beta 2-Glycoprotein I Immunoglobulin G Low-density lipoprotein Immunology Antibodies Antiphospholipid biology.protein lipids (amino acids peptides and proteins) Antibody business Lipoprotein |
| Zdroj: | Lupus. 16:929-938 |
| ISSN: | 1477-0962 0961-2033 |
| DOI: | 10.1177/0961203307084170 |
| Popis: | Several interpretations have been made regarding the specificity of antiphospholipid antibodies and antibodies against oxidized low-density lipoprotein (oxLDL), but these are still controversial. In the present study, we delineated specificity of these two types of antibodies and analyzed their regulatory effect on oxLDL and/or β2-glycoprotein I (β2GPI) binding to macrophages. Scavenger receptor-mediated binding of oxLDL (or its β2GPI complexes) to macrophages was observed and the binding was partly prevented by β2GPI. The IgG monoclonal anti-β2GPI antibody (WB-CAL-1), which was derived from NZW × BXSB F1 mouse (a model of antiphospholipid syndrome), significantly increased the oxLDL/β2GPI binding to macrophages. In contrast, IgM anti-oxLDL natural antibody, EO6 (derived from apoe-/-mouse), prevented the binding. Different antigenic specificity of these antibodies to oxLDL and its β2GPI complexes was also confirmed in TLC—ligand blot and ELISA. Thus, IgG anti-β2GPI autoantibodies contribute to lipid metabolism (housekeeping of oxLDL by macrophages) whereas IgM natural anti-oxLDL antibodies may protect against atherogenesis. In addition, in vitro data suggest that relatively high dose of intravenous immunoglobulin preparations (mainly contain IgG anti-oxLDL antibodies) might also prevent atherogenesis by inhibiting the oxLDL binding to macrophages. Lupus (2007) 16, 929—938. |
| Databáze: | OpenAIRE |
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