RC-6 ribonuclease induces caspase activation, cellular senescence and neuron-like morphology in NT2 embryonal carcinoma cells
| Autor: | Giou Teng Yiang, Liang Chih Liu, Tsai Kun Wu, Pei Lun Chou, Hsu Hung Tseng, Hsiao Chun Liu, Wei Jung Chang, Yung Luen Yu, Jer Rong Chen, Hsiu Feng Tsai |
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| Rok vydání: | 2013 |
| Předmět: |
Senescence
Cancer Research Embryonal Carcinoma Stem Cells Cell Blotting Western Fluorescent Antibody Technique Antineoplastic Agents Embryonal carcinoma Ribonucleases Carcinoma Embryonal Cell Line Tumor medicine Cytotoxic T cell Animals Humans Cytotoxicity Caspase Cellular Senescence Neurons biology Caspase 3 Cell Differentiation General Medicine Cell cycle medicine.disease Caspase 9 Cell biology Enzyme Activation medicine.anatomical_structure Oncology Cell culture Cancer research biology.protein Anura |
| Zdroj: | Oncology reports. 31(4) |
| ISSN: | 1791-2431 |
| Popis: | Frog ribonucleases have been demonstrated to have anticancer activities. However, whether RC-6 ribonuclease exerts anticancer activity on human embryonal carcinoma cells remains unclear. In the present study, RC-6 induced cytotoxicity in NT2 cells (a human embryonal carcinoma cell line) and our studies showed that RC-6 can exert anticancer effects and induce caspase-9 and -3 activities. Moreover, to date, there is no evidence that frog ribonuclease-induced cytotoxicity effects are related to cellular senescence. Therefore, our studies showed that RC-6 can increase p16 and p21 protein levels and induce cellular senescence in NT2 cells. Notably, similar to retinoic acid-differentiated NT2 cells, neuron-like morphology was found on some remaining live cells after RC-6 treatment. In conclusion, our study is the first to demonstrate that RC-6 can induce cytotoxic effects, caspase-9/-3 activities, cellular senescence and neuron-like morphology in NT2 cells. |
| Databáze: | OpenAIRE |
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