Effects of heat exposure on Akt/S6K1 signaling and expression of genes related to protein and energy metabolism in chicken (Gallus gallus) pectoralis major muscle
| Autor: | Nadia Everaert, Nicole Rideau, Sophie Tesseraud, R. Joubert, Cécile Berri, Sonia Métayer-Coustard, S. Temim, S. Boussaid-Om Ezzine, Anne Collin |
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| Přispěvatelé: | Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Department of Biosystems (BIOSYST), Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Ecole Nationale Supérieure Vétérinaire d'Alger (ENSV) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Hot Temperature Anabolism Physiology [SDV]Life Sciences [q-bio] Protein metabolism Gene Expression P70-S6 Kinase 1 Biology Carbohydrate metabolism Biochemistry Muscle hypertrophy Pectoralis Muscles Avian Proteins 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Animals Insulin [INFO]Computer Science [cs] Molecular Biology Protein kinase B 030304 developmental biology 2. Zero hunger 0303 health sciences 0402 animal and dairy science Skeletal muscle Ribosomal Protein S6 Kinases 70-kDa 04 agricultural and veterinary sciences 040201 dairy & animal science Endocrinology medicine.anatomical_structure chemistry Signal transduction Energy Metabolism Chickens Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Comparative Biochemistry and Physiology-Part B: Biochemistry and Molecular Biology Comparative Biochemistry and Physiology-Part B: Biochemistry and Molecular Biology, Elsevier, 2010, 157 (3), pp.281-287. ⟨10.1016/j.cbpb.2010.07.001⟩ |
| ISSN: | 1096-4959 |
| Popis: | International audience; In order to improve understanding of the heat-induced changes in muscle growth, we determined the expression of genes related to protein and energy metabolism in the pectoralis major muscle of chickens. We also explored the protein kinase B (PKB also called Akt)/p70 S6 kinase (S6K1)/S6 pathway that mediates anabolic signals thereby regulating metabolism and hypertrophic/atrophic balance. Four-week-old chickens were exposed to 32 or 22 degrees C for 1 week. Chickens from both groups were then fasted for 16 h or left fed, and submitted to an oral administration of glucose-arginine to induce an anabolic response (30-min treatment) or left untreated. High ambient temperature and the associated decrease in feed intake modified the expression of certain energy-related genes (e.g. -40% for PGC-1 alpha.) and protein metabolism (e.g. about +80% for atrogin-1), but the expression of several muscle metabolism-related genes considered here was unchanged. The capacity for muscle protein synthesis, i.e. RNA/protein ratio, was reduced in warm conditions (similar to-20%). Slightly lower activation of S6 induced by glucose-arginine treatment was found at 32 degrees C compared to 22 degrees C, which might indicate somewhat lower efficiency of mRNA translation. Analysis of glucose/insulin balance suggested changes in glucose metabolism under heat exposure. However, this remains to be characterized. (c) 2010 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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