The Third Revolution in Sequencing Technology
| Autor: | Delphine Naquin, Erwin van Dijk, Yan Jaszczyszyn, Claude Thermes |
|---|---|
| Přispěvatelé: | Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Plateforme de séquençage à haut débit (NGS), Département Plateforme (PF I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
[SDV]Life Sciences [q-bio] real-time dna methylation Computational biology Third generation sequencing Biology Genome DNA sequencing Structural variation 03 medical and health sciences symbols.namesake human genome Exome Sequencing Genetics Humans nanopore third-generation sequencing Sanger sequencing disease synthetic long-read sequencing structural variation High-Throughput Nucleotide Sequencing single-molecule real-time sequencing DNA Sequence Analysis DNA 030104 developmental biology long-read sequencing nanopore sequencing surveillance symbols n-6-adenine next-generation sequencing Human genome single-molecule Nanopore sequencing transcriptome Single molecule real time sequencing |
| Zdroj: | Trends in Genetics Trends in Genetics, 2018, 34 (9), pp.666--681. ⟨10.1016/j.tig.2018.05.008⟩ |
| ISSN: | 0168-9525 |
| DOI: | 10.1016/j.tig.2018.05.008⟩ |
| Popis: | International audience; Forty years ago the advent of Sanger sequencing was revolutionary as it allowed complete genome sequences to be deciphered for the first time. A second revolution came when next-generation sequencing (NGS) technologies appeared, which made genome sequencing much cheaper and faster. However, NGS methods have several drawbacks and pitfalls, most notably their short reads. Recently, third-generation/long-read methods appeared, which can produce genome assemblies of unprecedented quality. Moreover, these technologies can directly detect epigenetic modifications on native DNA and allow whole-transcript sequencing without the need for assembly. This marks the third revolution in sequencing technology. Here we review and compare the various long-read methods. We discuss their applications and their respective strengths and weaknesses and provide future perspectives. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect