Cloning of the V‐ATPase B subunit cDNA from Culex quinquefasciatus and expression of the B and C subunits in mosquitoes
| Autor: | Maria Filippova, L. S. Ross, Sarjeet S. Gill |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Vacuolar Proton-Translocating ATPases
Malpighian tubule system DNA Complementary animal structures Protein subunit Blotting Western Molecular Sequence Data Gene Expression Biology Aedes Complementary DNA parasitic diseases Genetics Animals Amino Acid Sequence Northern blot Cloning Molecular Molecular Biology Cloning chemistry.chemical_classification Base Sequence fungi Midgut Blotting Northern biology.organism_classification Molecular biology Culex quinquefasciatus Amino acid Culex Proton-Translocating ATPases chemistry Insect Science Rabbits |
| Zdroj: | Insect Molecular Biology. 7:223-232 |
| ISSN: | 1365-2583 0962-1075 |
| DOI: | 10.1111/j.1365-2583.1998.00069.x |
| Popis: | The V-ATPase B subunit cDNA isolated from the midgut and Malpighian tubules of Culex quinquefasciatus larvae has a 1476 bp ORF encoding a 492 amino acids protein with a predicted mass of 54.8 kDa. Northern blot analysis reveals the presence of 1.8 and 4.2 kb transcripts in larvae and a 3.0 kb transcript in pupae. A single 57 kDa protein band is detected in immunoblot analysis of protein extracts from C. quinquefasciatus and A. aegypti larvae. Using antibodies to the B subunits we demonstrate high-level expression of these subunits in ion-transporting cells of caeca and anterior midgut, and in Malpighian tubules and rectum. High V-ATPase expression was also observed in the larval salivary glands, central nervous system neurophile, the thoracic endocrine complex and in imaginal discs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|