A protective monoclonal anti-idiotypic vaccine to lethal Semliki Forest virus infection in BALB/c mice
| Autor: | T. A. M. Oosterlaken, Martin C. Harmsen, G. L. Ekstijn, C. A. Kraaijeveld, S. S. Jhagjhoor-Singh, Harm Snippe |
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| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.drug_class viruses Immunology Semliki Forest virus Monoclonal antibody Microbiology Immunoglobulin G BALB/c Immunoenzyme Techniques Mice L Cells Togaviridae Infections Immunoglobulin Idiotypes Species Specificity Neutralization Tests Virology medicine Animals Neutralizing antibody Mice Inbred BALB C biology Antibodies Monoclonal virus diseases Viral Vaccines biochemical phenomena metabolism and nutrition biology.organism_classification Semliki forest virus Anti-idiotypic vaccine Mice Inbred C57BL Immunization Mice Inbred DBA Insect Science Antibody Formation biology.protein Female Keyhole limpet hemocyanin Research Article |
| Zdroj: | Journal of Virology. 65:98-102 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.65.1.98-102.1991 |
| Popis: | Two monoclonal anti-idiotypic antibodies (ab2 MAbs), designated 1.13A112 (immunoglobulin G type 2a [IgG2a]) and 1.13A321 (IgG1), were prepared against Semliki Forest virus (SFV)-neutralizing ab1 MAb UM 1.13. They were identified in hybridoma supernatant fluid by their capacity to block UM 1.13-mediated neutralization of SFV. Although the neutralization-blocking capacities of the ab2 MAbs did not differ, only 1.13A321 evoked SFV-neutralizing ab3 antibodies upon intracutaneous and subcutaneous immunization of BALB/c mice with 1.13A321 chemically cross-linked to keyhole limpet hemocyanin and combined with the adjuvant Quil A. SFV-neutralizing ab3 antibodies appeared in serum within 10 days after primary immunization, and neutralizing antibody titers could be as high as 1/1,000 at day 35. All mice who had developed SFV-neutralizing antibodies upon anti-idiotypic immunization survived an otherwise lethal challenge with virulent SFV. However, induction of SFV-neutralizing ab3 antibodies by ab2 MAb 1.13A321 proved to be genetically restricted to BALB/c mice; even haplotype-identical (H-2d) DBA/2 mice did not respond, and consequently those animals died after infection with virulent SFV. |
| Databáze: | OpenAIRE |
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