Oral administration of lyophilized Dunaliella salina, a carotenoid-rich marine alga, reduces tumor progression in mammary cancer induced rats
Autor: | Kodiveri Muthukaliannan Gothandam, Jagannathan Sampathkumar Shravan, J. G. S. Pavan Kumar, Amballa Chaitanyakumar, Ramachandran Srinivasan, Anbazhagan Mageswari, Ajitha Gomathi, Mathiyazhagan Jayasindu, Gunasekaran Bharath |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
9 10-Dimethyl-1 2-benzanthracene DMBA Administration Oral Apoptosis Breast Neoplasms Pharmacology Biology Lipid peroxidation Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Oral administration Chlorophyta Benz(a)Anthracenes Animals Anticarcinogenic Agents Humans Rats Wistar Receptor Cell Proliferation Cell growth Caspase 3 Plant Extracts General Medicine biology.organism_classification Carotenoids Rats 030104 developmental biology chemistry Biochemistry Tumor progression 030220 oncology & carcinogenesis Dunaliella salina Disease Progression Female Lipid Peroxidation Food Science |
Zdroj: | Foodfunction. 8(12) |
ISSN: | 2042-650X |
Popis: | Dunaliella salina is a photosynthetic cell factory used for the commercial production of food additives, cattle stock feed and cosmetics as well as active ingredients for pharmaceutical industries. The investigation of the in vivo antitumor activity of D. salina lyophilized powder (DSLP) against 7,12-dimethylbenz(a)anthracene (DMBA) induced mammary carcinogenesis in female Wistar rats indicated a dose-dependent effect of DSLP. We studied the effect of DSLP at two different dosages of 500 and 1000 mg per kg bw on DMBA induced mammary cancer in rats by measuring the status of antioxidant enzymes, phase I and phase II detoxification enzymes, lipid peroxidation, and glycoconjugated proteins and by investigating the expression pattern of cell proliferation (Ki-67), hormonal receptor (ER, PR and HER2) status by immunohistochemical analysis, and apoptotic (caspase-3 and -9) and pro-inflammatory (COX-2) markers by colorimetric analysis. After 16 weeks of the study, we observed 100% tumor formation (including high tumor incidence and tumor volume) and a significant increase in the level of hormonal receptors, cell proliferation, and pro-inflammatory and apoptosis markers in tumor-bearing animals compared to the control. The oral administration of DSLP (1000 mg per kg bw) to the DMBA treated animals showed up to 83.4% reduction of tumors and effectively restored the levels of biochemical markers in the mammary tissues in addition to the downregulation of the expression of molecular markers. In conclusion, DSLP was found to show a chemopreventive effect against breast cancer induced in rats through the suppression of cell proliferation and the induction of apoptosis. |
Databáze: | OpenAIRE |
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