Uneven distribution of intracellular Cl− in rat hippocampal neurons
| Autor: | Chiyoko Inagaki, Masafumi Inoue, Mitsuyoshi Hara, Sumio Ohnishi, Yoshito Mikami, Tohru Yasukura |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Intracellular Fluid
Sodium-Potassium-Chloride Symporters Diaphragm pump Hippocampal formation Hippocampus Chlorides Furosemide medicine Animals Rats Wistar Bumetanide Ion transporter Neurons Chemistry General Neuroscience Transporter Dendrites Rats Electrophysiology Ethacrynic Acid nervous system Biochemistry Biophysics Carrier Proteins Cotransporter Intracellular medicine.drug |
| Zdroj: | Neuroscience Letters. 143:135-138 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/0304-3940(92)90250-b |
| Popis: | Electrophysiological observations of neurons suggest that perikarya and dendrites differ in local intracellular Cl- concentration ([Cl-]i), that has not been demonstrated yet. Regional [Cl-]i in cultured hippocampal neurons was estimated using a Cl(-)-sensitive fluorescent dye. Calibration showed that perikaryonic [Cl-]i was lower than dendritic [Cl-]i. Ethacrynic acid, an inhibitor of the outwardly directed Cl(-)-pump, increased the perikaryonic but not dendritic [Cl-]i. A decrease in [Cl-]i induced by furosemide or bumetanide, inhibitors of Na+/K+/2Cl- cotransporters, was more prominent in dendrites than in perikarya. These findings suggest that uneven distribution of Cl- is generated by the region-specific localization of these transporters. |
| Databáze: | OpenAIRE |
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