Methylation of SOCS3 in Myeloproliferative Neoplasms and Secondary Erythrocytosis/Thrombocythemia
| Autor: | Şefik Güran, Oral Nevruz, Salih Kozan, Cengiz Beyan, Deniz Torun, Muhterem Bahçe, Mesut Akyol |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
lcsh:Internal medicine
Myeloproliferative neoplasm Biology Pathogenesis Polycythemia vera Secondary erythrocytosis/thrombocythemia hemic and lymphatic diseases medicine SOCS1 SOCS3 Myelofibrosis lcsh:RC31-1245 Essential thrombocythemia lcsh:RC633-647.5 digestive oral and skin physiology Hematopoietic stem cell Hematology Methylation lcsh:Diseases of the blood and blood-forming organs medicine.disease medicine.anatomical_structure DNA methylation Immunology Cancer research Research Article |
| Zdroj: | Turkish Journal of Hematology, Vol 30, Iss 1, Pp 13-18 (2013) Turkish Journal of Hematology |
| ISSN: | 1308-5263 1300-7777 |
| Popis: | Objective: Myeloproliferative neoplasms (MPNs) like essential thrombocythemia (ET), polycythemia vera (PV), and primary myelofibrosis (PMF) are acquired clonal hematopoietic stem cell disorders and originate from a multipotent hematopoietic stem cell. The SOCS1 and SOCS3 genes are negative regulators of the JAK/STAT signal pathway. In this study we investigate the promoter methylation of these genes in the pathogenesis of MPNs and secondary erythrocytosis/thrombocythemia. Materials and Methods: Promoter methylation of SOCS1 and SOCS3 genes was analyzed with methylation-specific PCR. PCR products were analyzed by agarose gel electrophoresis. Results: No disease-specific CpG island methylation of SOCS1 was observed. Hypermethylation of the SOCS3 promoter was identified in 5 out of 19 (26.3%) PV cases, 2 out of 21 (9.5%) ET cases, 1 out of 5 (20%) PMF cases, and 9 out of 42 (21.4%) cases of secondary erythrocytosis/thrombocythemia. Conclusion: The results revealed that promoter methylation of the SOCS3 gene suggests a possible role for SOCS3 methylation in the pathogenesis of MPNs and secondary erythrocytosis/thrombocythemia. Conflict of interest:None declared. |
| Databáze: | OpenAIRE |
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