Immunology: CD56+ lymphoid cells in human first trimester pregnancy decidua as a source of novel transforming growth factor-β2-related immunosuppressive factors
| Autor: | Hal W. Hirte, Kathleen C. Flanders, G.S. Vince, P.M. Starkey, David A. Clark |
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| Rok vydání: | 1994 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte medicine.medical_specialty Lymphocyte chemical and pharmacologic phenomena Cell Separation Receptors Fc Biology Natural killer cell Immune tolerance Antigen Antigens CD Pregnancy Transforming Growth Factor beta Internal medicine Decidua Immune Tolerance medicine Humans Cytotoxic T cell Lymphocytes Rehabilitation Obstetrics and Gynecology hemic and immune systems Flow Cytometry Molecular biology CD56 Antigen Killer Cells Natural Pregnancy Trimester First medicine.anatomical_structure Endocrinology Reproductive Medicine Cell culture Female Neural cell adhesion molecule |
| Zdroj: | Human Reproduction. 9:2270-2277 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/oxfordjournals.humrep.a138436 |
| Popis: | The lymphomyeloid cells isolated from normal first trimester pregnancy decidua may be separated into a CD56+ population of natural killer (NK)-lineage cells with the morphology of granulated lymphocytes, and a CD56- population which includes other cell types. Unlike CD56+ NK cells in peripheral blood, decidual CD56+ cells lack type III Fc receptors (CD16) and did not express significant levels of either type I FcR (CD64) or type II FcR (CDw32). By contrast to the decidual CD56- cells, CD56+ cells could release biologically active transforming growth factor (TGF)-beta in vitro, detectable using an normal rat kidney fibroblast colony-forming assay. The CD56+ cells could be stained using an antibody specific for TGF-beta 2, and similarly staining cells could be detected in intact biopsies of normal pregnancy decidua. Bioactive TGF-beta is known to suppress the generation of cytotoxic cells in vitro, and high performance liquid chromatography fractionation of supernatants conditioned by CD56+ but not CD56- cells contained reproducible peaks of immunosuppressive activity at 40-45 and 15-20 kDa, similar to the TGF-beta 2 immunosuppressive activity in supernatants conditioned by unfractionated decidua. |
| Databáze: | OpenAIRE |
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