Characterization of a 3944 Da bacteriocin, produced by Enterococcus mundtii ST15, with activity against Gram-positive and Gram-negative bacteria
| Autor: | Leon M. T. Dicks, Svetoslav Dimitrov Todorov, M. De Kwaadsteniet, Hendriette Knoetze |
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| Rok vydání: | 2005 |
| Předmět: |
Time Factors
Propionibacterium Enterococcus mundtii Gram-positive bacteria Bacillus cereus Gram-Positive Bacteria Microbiology Enterococcus faecalis Bacteriocins Bacteriocin Antibiosis Gram-Negative Bacteria biology Temperature food and beverages General Medicine Hydrogen-Ion Concentration biology.organism_classification Clostridium tyrobutyricum Molecular Weight Enterococcus Food Microbiology Adsorption Food Science |
| Zdroj: | International Journal of Food Microbiology. 105:433-444 |
| ISSN: | 0168-1605 |
| DOI: | 10.1016/j.ijfoodmicro.2005.03.021 |
| Popis: | Strain ST15, isolated from soy beans, and identified as Enterococcus mundtii, produces a 3944 Da bacteriocin that inhibits the growth of Lactobacillus sakei, Enterococcus faecalis, Bacillus cereus, Propionibacterium sp., Clostridium tyrobutyricum, Acinetobacter baumanii, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae and Streptococcus caprinus. Bacteriocin ST15 is inactivated by proteinase K, pronase, pepsin, protease and Triton X-114, but not when treated with catalase, alpha-amylase, Triton X-100, SDS, Tween 20, Tween 80, urea and EDTA. No change in activity was recorded after 2 h at pH values between 2.0 and 12.0, and after treatment at 100 degrees C for 90 min. Activity was, however, lost after treatment at 121 degrees C for 20 min. The mode of activity is bactericidal. The highest level of activity (51200 AU ml(-1)) was recorded when cells were grown in MRS broth, pH 6.5. Bacteriocin ST15 differs from other broad-spectrum bacteriocins described for Enterococcus spp. by being active against Gram-negative bacteria and by being smaller. |
| Databáze: | OpenAIRE |
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