GLC Determination of Ibuprofen [dl-2-(p-Isobutylphenyl)propionic Acid] Enantiomers in Biological Specimens
| Autor: | Garrett J. Vangiesse, David G. Kaiser |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Adult
Male Residue (complex analysis) Chromatography Gas Time Factors Chromatography Elution Chemistry Extraction (chemistry) Pharmaceutical Science Ibuprofen Urine Middle Aged chemistry.chemical_compound Isomerism medicine Humans Racemic mixture Chromatography Thin Layer Propionates Enantiomer Benzene Half-Life medicine.drug |
| Zdroj: | Journal of Pharmaceutical Sciences. 64:798-801 |
| ISSN: | 0022-3549 |
| Popis: | To study the behavior of the d- and l-isomers of ibuprofen in humans, a method for the determination of the individual enantiomers in plasma and urine was required. A specific procedure was developed based on (a) benzene extraction of the acidified specimens, (b) TLC of the benzene extract residue, (c) formation of the l-alpha-methylbenzylamides of the materials eluted from the chromatograms, and (d) quantification of the resulting diastereoisomeric amides by GLC in conjunction with flame-ionization detection. When using a 1-ml aliquot of the specimen, the method is sensitive to 1 mug of each enantiomer/ml of plasma or urine. As compared to simple aqueous solutions, the average recoveries of the enantiomers from plasma and urine ranged from 94 to96%. Mass spectrometric analyses, in conjunction with GLC, confirmed the specificity of the method for the intact enantiomers. The procedure was applied successfully to drug absorption studies in humans. After oral administration of the racemic mixture, the predominant enantiomer in peripheral circulation and excreted in urine was of the d-configuration. |
| Databáze: | OpenAIRE |
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