FGF signaling promotes myoblast proliferation through activation of wingless signaling
| Autor: | Chandler Bragg, Maria B. Chechenova, Kumar Vishal, Richard M. Cripps, TyAnna L. Lovato |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Myoblast proliferation
Wnt1 Protein Fibroblast growth factor Article Myoblasts 03 medical and health sciences Imaginal wing disc 0302 clinical medicine biology.animal FGF Animals Drosophila Proteins Myocyte FGF Receptor Molecular Biology Cell Proliferation 030304 developmental biology 0303 health sciences Gene knockdown biology Proliferative capacity Cell Biology musculoskeletal system FGF Signaling Pathway Cell biology Fibroblast Growth Factors Drosophila melanogaster Imaginal Discs Wingless Armadillo Drosophila 030217 neurology & neurosurgery Signal Transduction Developmental Biology |
| Zdroj: | Developmental biology |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2020.05.009 |
| Popis: | Indirect flight muscles (IFMs) are the largest muscles in Drosophila and are made up of hundreds of myonuclei. The generation of these giant muscles requires a large pool of wing disc associated adult muscle precursors (AMPs), however the factors that control proliferation to form this myoblast pool are incompletely known. Here, we examine the role of fibroblast growth factor (FGF) signaling in the proliferation of wing disc associated myoblasts. We find that the components of FGF signaling are expressed in myoblasts and surrounding epithelial cells of the wing disc. Next, we show that attenuation of FGF signaling results in a diminished myoblast pool. This reduction in the pool size is due to decreased myoblast proliferation. By contrast, activating the FGF signaling pathway increases the myoblast pool size and restores the proliferative capacity of FGF knockdown flies. Finally, our results demonstrate that the FGF receptor Heartless acts through up-regulating β-catenin/Armadillo signaling to promote myoblast proliferation. Our studies identify a novel role for FGF signaling during IFM formation and uncover the mechanism through which FGF coordinates with Wingless signaling to promote myoblast proliferation. |
| Databáze: | OpenAIRE |
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