Assessment of diagnostic and analytic performance of the SD Bioline Dengue Duo test for dengue virus (DENV) infections in an endemic area (Savannakhet province, Lao People's Democratic Republic)

Autor: Christina Deschermeier, Herbert Schmitz, Vatsana Sopraseuth, Jessica Hansen, Simone Kann, Angela Mika, Yvonne Winkelmann, Petra Emmerich, Latdamone Keoviengkhone, Hussein El Halas, Jörg Blessmann, Jonas Schmidt-Chanasit
Rok vydání: 2020
Předmět:
RNA viruses
Male
0301 basic medicine
Viral Diseases
Physiology
viruses
Immune Complex
Viral Nonstructural Proteins
Dengue virus
Pathology and Laboratory Medicine
Antibodies
Viral
medicine.disease_cause
Biochemistry
Dengue Fever
Dengue fever
Dengue
0302 clinical medicine
Immune Physiology
Medicine and Health Sciences
Ethnicities
Enzyme-Linked Immunoassays
Immune System Proteins
Multidisciplinary
biology
Coinfection
Antibody titer
virus diseases
Endemic area
Middle Aged
Viral Load
Infectious Diseases
Medical Microbiology
Laos
Seroconversion
Viral Pathogens
Lao People
Viruses
Medicine
Female
Pathogens
Antibody
Viral load
Research Article
Neglected Tropical Diseases
Adult
Adolescent
Science
Point-of-Care Systems
Immunology
030231 tropical medicine
LAO PEOPLE'S DEMOCRATIC REPUBLIC
Enzyme-Linked Immunosorbent Assay
Research and Analysis Methods
Microbiology
Sensitivity and Specificity
Antibodies
Young Adult
03 medical and health sciences
Diagnostic Medicine
Virology
medicine
Humans
Immunoassays
Microbial Pathogens
Biology and life sciences
Flaviviruses
business.industry
Organisms
Proteins
Dengue Virus
biochemical phenomena
metabolism
and nutrition
Tropical Diseases
medicine.disease
Viral Disease Diagnosis
030104 developmental biology
Immunoglobulin M
Immune System
Immunoglobulin G
People and Places
Immunologic Techniques
biology.protein
Population Groupings
Reagent Kits
Diagnostic
business
Follow-Up Studies
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 3, p e0230337 (2020)
ISSN: 1932-6203
Popis: Background Rapid tests detecting both dengue virus (DENV) NS1 antigen and anti-DENV IgM and IgG antibodies facilitate diagnosis of dengue fever (DF) in resource-poor settings. Methodology/principal findings 92 acute phase serum samples from patients with a PCR-confirmed DENV infection collected in Lao People’s Democratic Republic (Lao PDR) in 2013 and 2015 were analyzed with the SD Bioline Dengue Duo test. A subset of 74 samples was additionally tested with the Platelia NS1 antigen test, the Panbio DENV μ-capture ELISA and the Panbio DENV IgG ELISA. IgM seroconversion was assayed using follow-up samples of 35 patients collected in the convalescent phase. 57.6%, 22.8% and 44.6% of acute phase serum samples tested positive in the SD Bioline Dengue Duo NS1, IgM, and IgG test, respectively. Diagnostic sensitivity of the SD Bioline Dengue Duo NS1 test strongly correlated with viral load, decreased rapidly over the acute phase of the disease, and was significantly reduced in presence of high anti-DENV IgG antibody titers resulting from secondary DENV infection. While a good concordance (Cohen’s kappa 0.78) was found between the SD Bioline Dengue Duo NS1 test and the Platelia NS1 antigen ELISA, both the SD Bioline Dengue Duo IgM and IgG test displayed a significantly lower sensitivity than the corresponding ELISA tests. Conclusions/significance The SD Bioline Dengue Duo test is a valuable tool for diagnosis of DENV infections especially when analyzing early acute phase samples with high viral load. Nevertheless, in endemic areas, where secondary flavivirus infections are common, diagnostic sensitivity of the NS1 and IgM test components may be compromised.
Databáze: OpenAIRE
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