Nanogold-functionalized g-C3N4 nanohybrids for sensitive impedimetric immunoassay of prostate-specific antigen using enzymatic biocatalytic precipitation
| Autor: | Ya Xiong, Lili Ding, Zhengyu Zhang, Jing-Ping Ge, Wen-Quan Zhou, Jianping Gao |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Faradaic impedance
Biomedical Engineering Biophysics Metal Nanoparticles Biosensing Techniques 02 engineering and technology 01 natural sciences Horseradish peroxidase Antibodies Nanocomposites Antigen Limit of Detection Nitriles Electric Impedance Electrochemistry medicine Humans Horseradish Peroxidase Immunoassay Detection limit Chromatography medicine.diagnostic_test biology Chemistry 010401 analytical chemistry Electrochemical Techniques General Medicine Prostate-Specific Antigen 021001 nanoscience & nanotechnology 0104 chemical sciences Linear range Polyclonal antibodies Electrode biology.protein Graphite Gold 0210 nano-technology Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 85:212-219 |
| ISSN: | 0956-5663 |
| Popis: | This work reports on a new impedimetric immunosensing strategy for sensitive detection of prostate-specific antigen (PSA) in biological fluids. The assay was carried out on monoclonal anti-PSA capture antibody-modified glassy carbon electrode with a sandwich-type detection format. Gold nanoparticles-decorated g-C3N4 nanosheets (AuNP/g-C3N4), synthesized by the wet-chemistry method, were utilized for the labeling of polyclonal anti-PSA detection antibody and horseradish peroxidase (HRP). Upon target PSA introduction, the sandwiched immunocomplex could be formed between capture antibody and detection antibody. Followed by the AuNP/g-C3N4, the labeled HRP could catalyze 4-choloro-1-naphthol into benzo-4-chlorohexadienone. The as-generated insoluble product was coated on the electrode surface, thus increasing the Faradaic impedance of Fe(CN)6(4-/3)(-) indicator between the solution and the base electrode. Under the optimal conditions, the impedance increased with the increasing target PSA in the sample, and exhibited a wide linear range from 10pgmL(-1) and 30ngmL(-1) with a detection limit of 5.2pgmL(-1). A repeatability and intermediate precision of |
| Databáze: | OpenAIRE |
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