A microfluidic platform for drug screening in a 3D cancer microenvironment
| Autor: | Hadi Shafiee, Devbalaji Prabhakar, Hardik J. Pandya, Karan Dhingra, Anish Vasan, Ashish Kulkarni, Siva Kumar Natarajan, Vineethkrishna Chandrasekar |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Drug Male media_common.quotation_subject Microfluidics Biomedical Engineering Biophysics Cell Culture Techniques Drug Evaluation Preclinical Breast Neoplasms Drug resistance Biosensing Techniques Pharmacology Article 03 medical and health sciences Prostate cancer Mice Breast cancer Electrochemistry Tumor Microenvironment Medicine Animals Humans media_common Tumor microenvironment business.industry Melanoma Cancer Prostatic Neoplasms General Medicine medicine.disease 030104 developmental biology Drug Resistance Neoplasm Cancer cell Cancer research Female business Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 94 |
| ISSN: | 1873-4235 |
| Popis: | Development of resistance to chemotherapy treatments is a major challenge in the battle against cancer. Although a vast repertoire of chemotherapeutics is currently available for treating cancer, a technique for rapidly identifying the right drug based on the chemo-resistivity of the cancer cells is not available and it currently takes weeks to months to evaluate the response of cancer patients to a drug. A sensitive, low-cost diagnostic assay capable of rapidly evaluating the effect of a series of drugs on cancer cells can significantly change the paradigm in cancer treatment management. Integration of microfluidics and electrical sensing modality in a 3D tumour microenvironment may provide a powerful platform to tackle this issue. Here, we report a 3D microfluidic platform that could be potentially used for a real-time deterministic analysis of the success rate of a chemotherapeutic drug in less than 12 h. The platform (66 mm × 50 mm; L×W) is integrated with the microsensors (interdigitated gold electrodes with width and spacing 10 μm) that can measure the change in the electrical response of cancer cells seeded in a 3D extra cellular matrix when a chemotherapeutic drug is flown next to the matrix. B16-F10 mouse melanoma, 4T1 mouse breast cancer, and DU 145 human prostate cancer cells were used as clinical models. The change in impedance magnitude on flowing chemotherapeutics drugs measured at 12 h for drug-susceptible and drug tolerant breast cancer cells compared to control were 50552 ± 144 Ω and 28786 ± 233 Ω, respectively, while that of drug-susceptible melanoma cells were 40197 ± 222 Ω and 4069 ± 79 Ω, respectively. In case of prostate cancer the impedance change between susceptible and resistant cells were 8971 ± 1515 Ω and 3281 ± 429 Ω, respectively, which demonstrated that the microfluidic platform was capable of delineating drug susceptible cells, drug tolerant, and drug resistant cells in less than 12 h. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect