Effects of Exogenous Salicylic Acid on Ganoderic Acid Biosynthesis and the Expression of Key Genes in the Ganoderic Acid Biosynthesis Pathway in the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes)
| Autor: | Mingwen Zhao, Chen-Gao Wu, Liang Shi, Ang Ren, Zhi-Hao Dang, Ai-Liang Jiang, Peng-Fei Cao |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pharmacology Reishi Lanosterol Gene Expression Profiling Ganoderic acid Reductase Applied Microbiology and Biotechnology Triterpenes Biosynthetic Pathways Terpene 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Biochemistry Biosynthesis Gene Expression Regulation Drug Discovery Gene expression Medicinal fungi Salicylic Acid Salicylic acid |
| Zdroj: | International journal of medicinal mushrooms. 19(1) |
| ISSN: | 1940-4344 |
| Popis: | We demonstrate herein that salicylic acid (SA) can enhance ganoderic acid (GA) accumulation in the lingzhi or reishi medicinal mushroom Ganoderma lucidum. Following treatment with different concentrations of SA, the GA content was increased 22.72% to 43.04% compared with the control group. When the fungi were treated with 200 μmol/L SA at different times, the GA content was improved 10.21% to 35.24% compared with the control group. By choosing the optimum point based on response surface methodology, the GA content could be increased up to 229.03 μg/100 mg, which was improved 66.38% compared with the control group. When the fungi were treated with 200 μmol/L SA, the transcription levels of key genes in the GA biosynthesis pathway-squalene (SQ) synthase (sqs), lanosterol (Lano; osc), and hydroxy-3-methylglutaryl-coenzyme A reductase (hmgr)-were improved 119.6-, 3.2-, and 4.2-fold, respectively. In addition, following treatment with 100 μmol/L SA, the levels of Lano and SQ, which are intermediate metabolites of GA biosynthesis, were increased 2.8- and 1.4-fold, respectively. These results indicate that SA can regulate the expression of genes related to GA biosynthesis and increases the metabolic levels of Lano and SQ, thereby resulting in the accumulation of GA. |
| Databáze: | OpenAIRE |
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