Validation of mathematical model with phosphate activation effect by batch (R)-phenylacetylcarbinol biotransformation process utilizing Candida tropicalis pyruvate decarboxylase in phosphate buffer
| Autor: | Thanongsak Chaiyaso, Sumeth Sommanee, Ngoc Thao Ngan Trinh, Noppol Leksawasdi, Phisit Seesuriyachan, Kritsadaporn Porninta, Julaluk Khemacheewakul, Sutee Wangtueai, Alissara Reungsang, Sarana Rose Sommano, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Chatchadaporn Mahakuntha, Siraphat Taesuwan, Charin Techapun, Rojarej Nunta, Yuthana Phimolsiripol |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Science 030106 microbiology Kinetics 01 natural sciences Microbiology Article Candida tropicalis Benzaldehyde Applied microbiology 03 medical and health sciences chemistry.chemical_compound Reaction rate constant Engineering Chemical engineering Biotransformation 010608 biotechnology Multidisciplinary Chromatography biology biology.organism_classification Phosphate Phenylacetylcarbinol chemistry Medicine Pyruvate decarboxylase Biotechnology |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
| ISSN: | 2045-2322 |
| Popis: | The (R)-phenylacetylcarbinol (PAC) batch biotransformation kinetics for partially purified Candida tropicalis TISTR 5350 pyruvate decarboxylase (PDC) were determined to validate a comprehensive mathematical model in 250 mL scale with 250 mM phosphate buffer/pH 7.0. PDC could convert initial 100/120 mM benzaldehyde/pyruvate substrates to the statistical significantly highest (p ≤ 0.05) maximum PAC concentration (95.8 ± 0.1 mM) and production rate (0.639 ± 0.001 mM min−1). A parameter search strategy aimed at minimizing overall residual sum of square (RSST) based on a system of six ordinary differential equations was applied to PAC biotransformation profiles with initial benzaldehyde/pyruvate concentration of 100/120 and 30/36 mM. Ten important biotransformation kinetic parameters were then elucidated including the zeroth order activation rate constant due to phosphate buffer species (ka) of (9.38 ± –6% relative PDC activity min−1 mM−1. The validation of this model to independent biotransformation kinetics with initial benzaldehyde/pyruvate concentration of 50/60 mM resulted in relatively good fitting with RSST, mean sum of square error (MSE), and coefficient of determination (R2) values of 662, 17.4, and 0.9863, respectively. |
| Databáze: | OpenAIRE |
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