Volatile Organic Compounds (VOCs) Produced by Gluconobacter cerinus and Hanseniaspora osmophila Displaying Control Effect against Table Grape-Rot Pathogens
| Autor: | Ximena Besoain, Ninoska Delgado, Eduardo Salgado, Claudia Fuentealba, Romina Pedreschi, Fabiola Cádiz, Alejandro Madrid, Iván Montenegro, Guillermo Bravo, Matías Olivera |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) food.ingredient 030106 microbiology Ethyl acetate RM1-950 Biochemistry Microbiology Red Globe Article 03 medical and health sciences chemistry.chemical_compound food summer bunch rot volatile organic compounds Pharmacology (medical) biocontrol General Pharmacology Toxicology and Pharmaceutics Mycelium Botrytis cinerea Botrytis biology Chemistry Table grape fungi food and beverages table grapes biology.organism_classification Spore Horticulture 030104 developmental biology Infectious Diseases mycelial inhibition Therapeutics. Pharmacology Penicillium expansum bioantagonist |
| Zdroj: | Antibiotics Volume 10 Issue 6 Antibiotics, Vol 10, Iss 663, p 663 (2021) |
| ISSN: | 2079-6382 |
| DOI: | 10.3390/antibiotics10060663 |
| Popis: | Table grapes (Vitis vinifera) are affected by botrytis bunch rot and summer bunch rot, the latter a complex disease caused by Botrytis cinerea, Aspergillus spp., Penicillium expansum and Rhizopus stolonifer. To search for biocontrol alternatives, a new bioproduct composed of Gluconobacter cerinus and Hanseniaspora osmophila, a consortium called PUCV-VBL, was developed for the control of fungal rots in table grapes. Since this consortium presents new biocontrol species, the effect of their VOCs (volatile organic compounds) was evaluated under in vitro and in vivo conditions. The VOCs produced by the PUCV-VBL consortium showed the highest mycelial inhibition against Botrytis cinerea (86%). Furthermore, H. osmophila was able to inhibit sporulation of A. tubingensis and P. expansum. VOCs’ effect in vivo was evaluated using berries from Red Globe, Thompson Seedless and Crimson Seedless grapes cultivars, demonstrating a mycelial inhibition by VOCs greater than 70% for all evaluated fungal species. The VOC identification of the PUCV-VBL consortium was analyzed by solid-phase microextraction coupled to gas chromatography-mass spectrometry (SPME-GCMS). A total 26 compounds were identified, including 1-butanol 3-methyl, propanoic acid ethyl ester, ethyl acetate, phenylethyl alcohol, isobutyl acetate and hexanoic acid ethyl ester. Our results show that VOCs are an important mode of action of the PUCV-VBL biological consortium. |
| Databáze: | OpenAIRE |
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