Characterization of replication defects induced by mutations in the basic domain and C-terminus of HIV-1 matrix
| Autor: | Antonito T. Panganiban, Nancy Campbell, Ajay K. Bhatia, Lee Ratner |
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| Rok vydání: | 2007 |
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Molecular HIV Antigens Mutant Mutagenesis (molecular biology technique) Biology medicine.disease_cause Virus Replication gag Gene Products Human Immunodeficiency Virus Article Cell Line Viral envelope Viral entry Virology HIV-1 Matrix medicine Humans Fusion Sequence Deletion Infectivity Mutation C-terminus Amino Acids Basic Virus Assembly Envelope Incorporation Virion env Gene Products Human Immunodeficiency Virus Virus Internalization Molecular biology HIV Envelope Protein gp41 Protein Structure Tertiary Viral replication HIV-1 |
| Zdroj: | Virology. 369(1) |
| ISSN: | 0042-6822 |
| Popis: | Extensive mutagenesis has defined distinct functional domains in the HIV-1 matrix domain (MA). In an attempt to more clearly define functions of regions of MA which affect viral entry, we analyzed mutations in the N-terminal basic and the C-terminal helical domains. Deletions of 8–10 amino acid residues of the C-terminal fifth helix of MA resulted in viruses that were only mildly defective in infectivity and fusion. The defect exhibited by these mutations could largely be attributed to a reduction in levels of viral envelope incorporated into mature virions. Truncation of the gp41 cytoplasmic tail (gp41CT) could rescue the phenotype of one of these mutants. In contrast, mutations of multiple basic residues in the N-terminus of MA were severely defective in both infectivity and fusion. While these mutations induce severe envelope incorporation defects, they also result in virus crippled at a post-entry step, since truncation of the gp41CT could not rescue the infectivity defect. |
| Databáze: | OpenAIRE |
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