Evidence for extensive genetic diversity and substructuring of the Babesia bovis metapopulation
| Autor: | Leonhard Schnittger, P. Rolls, Y. Minichiello, Flábio R. Araújo, M. Petterson, Daniel Benitez, Monica Florin-Christensen, G. M. Pacheco, Ignacio Echaide, Daniela Flores, V. Shkap, Juan Mosqueda |
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| Rok vydání: | 2012 |
| Předmět: |
Genetic Markers
Linkage disequilibrium POPULATION STRUCTURE Genotype Turkey 030231 tropical medicine Population SATELLITE MARKERS Argentina Zoology Cattle Diseases Biology BOVINE BABESIOSIS Variación Genética Disease Outbreaks Ciencias Biológicas 03 medical and health sciences 0302 clinical medicine Estructura de la Población Babesiosis Genetic variation Animals education 030304 developmental biology 2. Zero hunger Genetics MULTILOCUS TYPING 0303 health sciences education.field_of_study Genetic diversity General Veterinary General Immunology and Microbiology Genetic Variation Babesia bovis General Medicine Bioquímica y Biología Molecular biology.organism_classification BABESIA BOVIS 3. Good health Marcadores Genéticos Minisatellite Genetic marker GENETIC DIVERSITY Cattle CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Transboundary and Emerging Diseases 60 (s2) : 131–136 (November 2013) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA |
| ISSN: | 1865-1682 |
| Popis: | Babesia bovis is a tick-transmitted haemoprotozoan and a causative agent of bovine babesiosis, a cattle disease that causes significant economic loss in tropical and subtropical regions. A panel of nineteen micro- and minisatellite markers was used to estimate population genetic parameters of eighteen parasite isolates originating from different continents, countries and geographic regions including North America (Mexico, USA), South America (Argentina, Brazil), the Middle East (Israel) and Australia. For eleven of the eighteen isolates, a unique haplotype was inferred suggesting selection of a single genotype by either in vitro cultivation or amplification in splenectomized calves. Furthermore, a high genetic diversity (H = 0.780) over all marker loci was estimated. Linkage disequilibrium was observed in the total study group but also in sample subgroups from the Americas, Brazil, and Israel and Australia. In contrast, corresponding to their more confined geographic origin, samples from Israel and Argentina were each found to be in equilibrium suggestive of random mating and frequent genetic exchange. The genetic differentiation (FST) of the total study group over all nineteen loci was estimated by analysis of variance (Θ) and Nei's estimation of heterozygosity (GST') as 0.296 and 0.312, respectively. Thus, about 30% of the genetic diversity of the parasite population is associated with genetic differences between parasite isolates sampled from the different geographic regions. The pairwise similarity of multilocus genotypes (MLGs) was assessed and a neighbour-joining dendrogram generated. MLGs were found to cluster according to the country/continent of origin of isolates, but did not distinguish the attenuated from the pathogenic parasite state. The distant geographic origin of the isolates studied allows an initial glimpse into the large extent of genetic diversity and differentiation of the B. bovis population on a global scale. Fil: Flores, Daniela Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Minichiello, Y.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Araujo, F. R.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Shkap, V.. Kimron Veterinary Institute Israel; Israel Fil: Benítez, D.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Corrientes. Estación Experimental Agropecuaria Mercedes; Argentina Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina Fil: Rolls, P.. Tick Fever Centre; Australia Fil: Mosqueda, J.. Universidad Autónoma de Querétaro; México Fil: Pacheco, G. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Petterson, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Jacobsen, Monica Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina |
| Databáze: | OpenAIRE |
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