Diet assessment of the Atlantic Sea Nettle Chrysaora quinquecirrha in Barnegat Bay, New Jersey, using next-generation sequencing
| Autor: | Paul A.X. Bologna, John J. Gaynor, Robert W. Meredith |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Food Chain 010603 evolutionary biology 01 natural sciences Chrysaora quinquecirrha Predation Food chain Genetics Animals Ecology Evolution Behavior and Systematics Polychaete geography geography.geographical_feature_category biology New Jersey 010604 marine biology & hydrobiology High-Throughput Nucleotide Sequencing Estuary Sea Nettle East Coast Plankton biology.organism_classification Shrimp Diet Fishery Bays Predatory Behavior Bay |
| Zdroj: | Molecular ecology. 25(24) |
| ISSN: | 1365-294X |
| Popis: | Next-generation sequencing (NGS) methodologies have proven useful in deciphering the food items of generalist predators, but have yet to be applied to gelatinous animal gut and tentacle content. NGS can potentially supplement traditional methods of visual identification. Chrysaora quinquecirrha (Atlantic sea nettle) has progressively become more abundant in Mid-Atlantic United States' estuaries including Barnegat Bay (New Jersey), potentially having detrimental effects on both marine organisms and human enterprises. Full characterization of this predator's diet is essential for a comprehensive understanding of its impact on the food web and its management. Here, we tested the efficacy of NGS for prey item determination in the Atlantic sea nettle. We implemented a NGS 'shotgun' approach to randomly sequence DNA fragments isolated from gut lavages and gastric pouch/tentacle picks of eight and 84 sea nettles, respectively. These results were verified by visual identification and co-occurring plankton tows. Over 550 000 contigs were assembled from ~110 million paired-end reads. Of these, 100 contigs were confidently assigned to 23 different taxa, including soft-bodied organisms previously undocumented as prey species, including copepods, fish, ctenophores, anemones, amphipods, barnacles, shrimp, polychaete worms, flukes, flatworms, echinoderms, gastropods, bivalves and hemichordates. Our results not only indicate that a 'shotgun' NGS approach can supplement visual identification methods, but targeted enrichment of a specific amplicon/gene is not a prerequisite for identifying Atlantic sea nettle prey items. |
| Databáze: | OpenAIRE |
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