Doxycycline suppresses Chlamydia pneumoniae induced interferon-gamma responses in peripheral blood mononuclear cells in children with allergic asthma
| Autor: | Margaret R. Hammerschlag, Rauno Joks, Stephan Kohlhoff, Helen G. Durkin, Diana Weaver, Tamar A. Smith-Norowitz, Yitzchok M. Norowitz |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) Adolescent 030106 microbiology Azithromycin medicine.disease_cause Immunoglobulin E Peripheral blood mononuclear cell Interferon-gamma Young Adult 03 medical and health sciences 0302 clinical medicine Ciprofloxacin medicine Humans Pharmacology (medical) Interferon gamma 030212 general & internal medicine Child Chlamydophila Infections Doxycycline Chlamydia biology business.industry Chlamydophila pneumoniae medicine.disease Asthma Anti-Bacterial Agents Mycoplasma pneumoniae Infectious Diseases Immunology Leukocytes Mononuclear biology.protein Female Interleukin-4 Antibody business medicine.drug |
| Zdroj: | Journal of Infection and Chemotherapy. 24:470-475 |
| ISSN: | 1341-321X |
| Popis: | Persistent respiratory infections caused by Chlamydia pneumoniae have been implicated in the pathogenesis of chronic diseases (e.g. asthma). Antibiotics are used to treat C. pneumoniae respiratory infections; however, the use of antibiotics as anti-inflammatory agents in treatment of asthma remains controversial. The current study investigated whether ciprofloxacin, azithromycin, or doxycycline can suppress C. pneumoniae-induced production of immunoglobulin (Ig) E or cytokines in peripheral blood mononuclear cells (PBMC) obtained from asthmatic children. Apart from blood, nasopharyngeal swab specimens were also collected to test for the presence of C. pneumoniae and/or M. pneumoniae (qPCR). PBMC (1.5 x 106) from asthmatic pediatric patients (N = 18) were infected or mock infected for 1 h ± C. pneumoniae AR-39 at a multiplicity of infection (MOI) = 0.1, and cultured ± ciprofloxacin, azithromycin, or doxycycline (0.1 or 1.0 μg/mLmL) for either 48 h (cytokines) or 10 days (IgE). Interleukin (IL)-4, interferon (IFN)-γ and IgE levels in supernatants were measured (ELISA). When PBMC were infected with C. pneumoniae, IL-4 and IFNγ production increased (p = 0.06 and 0.03, respectively); IgE levels were low. The now-elevated levels of IL-4 didn't decrease significantly after addition of ciprofloxacin, azithromycin, or doxycycline. However, infected PBMC IFNγ formation decreased significantly when 0.1 μg/mL doxycycline was employed (p = 0.04); no dose of ciprofloxacin or azithromycin had any impact. This inhibitory outcome with doxycycline lends support to the use of tetracyclines as immune modulators and anti-inflammatory medications in treatment of C. pneumoniae-infected asthma patients. |
| Databáze: | OpenAIRE |
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