Platinum Nanoparticles Enhance Exosome Release in Human Lung Epithelial Adenocarcinoma Cancer Cells (A549): Oxidative Stress and the Ceramide Pathway are Key Players
| Autor: | Min-Hee Kang, Jin-Hoi Kim, Muniyandi Jeyaraj, Sangiliyandi Gurunathan |
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| Rok vydání: | 2021 |
| Předmět: |
platinum nanoparticle
Serum Lung Neoplasms Metal Nanoparticles Pharmaceutical Science 02 engineering and technology Exosomes 01 natural sciences chemistry.chemical_compound International Journal of Nanomedicine Drug Discovery oxidative stress Original Research Aniline Compounds Chemistry General Medicine 021001 nanoscience & nanotechnology Neoplasm Proteins Cell biology Gene Expression Regulation Neoplastic Sphingomyelin Phosphodiesterase acetylcholinesterase activity Acetylcholinesterase cytotoxicity 0210 nano-technology Ceramide Cell Survival Static Electricity Biophysics Nanoparticle tracking analysis Adenocarcinoma of Lung Bioengineering Ceramides 010402 general chemistry Benzylidene Compounds Exosome Biomaterials exosome Humans Secretion RNA Messenger Particle Size Cell Proliferation Platinum A549 cell neutral sphingomyelinase activity Lutein Organic Chemistry Microvesicles Acetylcysteine 0104 chemical sciences Enzyme Activation A549 Cells Cancer cell Biogenesis |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| DOI: | 10.2147/ijn.s291138 |
| Popis: | Sangiliyandi Gurunathan, Min-Hee Kang, Muniyandi Jeyaraj, Jin-Hoi Kim Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, KoreaCorrespondence: Sangiliyandi Gurunathan; Jin-Hoi KimDepartment of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, KoreaTel +82 2 450 3687Fax + 82 2 544 4645Email gsangiliyandi@yahoo.com; jhkim541@konkuk.ac.krBackground: Several studies have demonstrated various molecular mechanisms involved in the biogenesis and release of exosomes. However, how external stimuli, such as platinum nanoparticles (PtNPs), induces the biogenesis and release of exosomes remains unclear. To address this, PtNPs were synthesized using lutein to examine their effect on the biogenesis and release of exosomes in human lung epithelial adenocarcinoma cancer cells (A549).Methods: The size and concentration of isolated exosomes were characterized by dynamic light scattering (DLS) and nanoparticle tracking analysis system (NTA). Morphology and structure of exosomes were examined using scanning electron microscopy and transmission electron microscopy (TEM), respectively. Quantification of exosomes were analyzed by EXOCETTM assay and fluorescence polarization (FP). The expression of typical markers of exosomes were analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA).Results: A549 cells cultured with PtNPs enhance exosome secretion by altering various physiological processes. Interestingly, A549 cells treated with PtNPs increases total protein concentration, biogenesis and release of exosomes associated with PtNPs-induced oxidative stress. GW4869 inhibits PtNPs induced biogenesis and release of exosomes and also acetylcholinesterase (AChE), neutral sphingomyelinase activity (n-SMase), and exosome counts. A549 cells pre-treated with N-acetylcysteine (NAC) significantly inhibited PtNPs induced exosome biogenesis and release. These findings confirmed that PtNPs-induced exosome release was due to the induction of oxidative stress and the ceramide pathway. These factors enhanced exosome biogenesis and release and may be useful in understanding the mechanism of exosome formation, release, and function.Conclusion: PtNPs provide a promising agent to increase exosome production in A549 cells. These findings offer novel strategies for enhancing exosome release, which can be applied in the treatment and prevention of cancer. Importantly, this is the first study, to our knowledge, showing that PtNPs stimulate exosome biogenesis by inducing oxidative stress and the ceramide pathway.Keywords: exosome, platinum nanoparticle, cytotoxicity, oxidative stress, acetylcholinesterase activity, neutral sphingomyelinase activity |
| Databáze: | OpenAIRE |
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