Rapid detection of Staphylococcus aureus and methicillin resistance in bone and joint infection samples: evaluation of the GeneXpert MRSA/SA SSTI assay
| Autor: | A. M. Freydière, Florent Valour, Frédéric Laurent, Anissa Bouaziz, Emmanuel Chanard, Tristan Ferry, Sébastien Lustig, Viviane Blanc-Pattin |
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| Přispěvatelé: | INSERM U1111, International Center for Research in Infectiology, Laboratory of bacteriology, North Lyon University Hospital, Hospices Civils de Lyon, Lyon, France, parent, Laboratory of bacteriology, East Lyon University Hospital, Hospices Civils de Lyon, Lyon, France, Department of infectious diseases, North Lyon University Hospital, Hospices Civils de Lyon, Lyon, France, Novescia, Laboratoire de Biomécanique et Mécanique des Chocs (LBMC UMR T9406), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR), French National Reference Center for Staphylococci, Hospices Civils de Lyon, Lyon, France |
| Rok vydání: | 2014 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Microbiology (medical) Staphylococcus aureus METHICILLIN-RESISTANCE REAL TIME POLYMERASE CHAIN REACTION medicine.disease_cause Rapid detection Methicillin resistance Microbiology BIOMECANIQUE 03 medical and health sciences 0302 clinical medicine GENEXPERT MRSA/SA SSTI ASSAY Humans Medicine 030212 general & internal medicine Arthritis Infectious Bacteriological Techniques 0303 health sciences GeneXpert MTB/RIF 030306 microbiology business.industry OS [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph] General Medicine Staphylococcal Infections Virology Glycopeptide 3. Good health Infectious Diseases Real-time polymerase chain reaction BONE AND JOINT INFECTION Bone Diseases business |
| Zdroj: | Diagnostic Microbiology and Infectious Disease Diagnostic Microbiology and Infectious Disease, Elsevier, 2014, 78 (3), pp. 313-315. ⟨10.1016/j.diagmicrobio.2013.11.026⟩ |
| ISSN: | 0732-8893 |
| Popis: | The GeneXpert MRSA/SA SSTI assay was compared to conventional cultures to detect Staphylococcus aureus and methicillin-resistance from 91 bone and joint infection samples. Sensitivity and specificity were 94.4% and 100%. Three false-positive results were observed, in fact providing from patients known to be infected by S. aureus on the basis of other concomitant osteoarticular samples, which suggests that PCR was more sensitive than culture. This diagnosis accuracy may help shorten toxic and non-optimal empirical therapies such as glycopeptides in case of methicillin-susceptible strains. |
| Databáze: | OpenAIRE |
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