Immunohistochemistry of carbonic anhydrase in human placenta and fetal membranes
| Autor: | Peter Kaufmann, M. Castellucci, Milovanov Ap, Seppo Parkkila, C. Crescimanno, J Mühlhauser, Hannu Rajaniemi |
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| Rok vydání: | 1994 |
| Předmět: |
Histology
Placenta Blotting Western Extraembryonic Membranes Umbilical cord Umbilical Cord Andrology Syncytiotrophoblast Fetal membrane Pregnancy Carbonic anhydrase medicine Humans Amnion Molecular Biology reproductive and urinary physiology Carbonic Anhydrases Fetus biology Cell Biology General Medicine Intervillous space Chorion Immunohistochemistry Isoenzymes Medical Laboratory Technology medicine.anatomical_structure embryonic structures Immunology biology.protein Female Anatomy General Agricultural and Biological Sciences |
| Zdroj: | Histochemistry. 101(2) |
| ISSN: | 0301-5564 |
| Popis: | The localization of human carbonic anhydrase (CA) isoenzymes HCA I, HCA II, and rat CA II have been studied in human umbilical cord, chorion laeve including amnion and placenta from first and second trimester and also from term pregnancies. Detection techniques of immunofluorescence and immunoperoxidase were used in cryostat and paraffin sections. Both isoenzymes were found in the villous syncytiotrophoblast throughout pregnancy. HCA I staining patterns in the villous endothelium were highly variable whereas increasing immunoreactivity levels of endothelial HCA II were detected as pregnancy advances. The extravillous cytotrophoblast showed generally weaker levels of immunoreactivity. In amnionic epithelium of membranes, chorionic plate and umbilical cord, higher activities for HCA I, HCA II and rat CA II were found than in all other localizations. Our findings emphasize the importance of enzyme mediated bicarbonate/CO2 removal from the feto-placental unit as opposed to simple bicarbonate diffusion or carrier mediated transport. As effective transfer routes should be considered not only umbilical cord — placental villi — intervillous space, but also fetal kidney — amnionic fluid — amnion — uterine vessels. |
| Databáze: | OpenAIRE |
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