Decreased FHIT protein expression correlates with a worse prognosis in patients with diffuse large B-cell lymphoma
| Autor: | Po Min Chen, Chueh-Chuan Yen, Tzeon Jye Chiou, Wei-Shu Wang, Ta-Chung Chao, Jin-Hwang Liu, Liang Tsai Hsiao, Chiau-Jun Chu, I-Ting Yu, Muh Hwa Yang |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Cancer Research Tumor suppressor gene Adolescent Transcription Genetic T-Lymphocytes Biology FHIT hemic and lymphatic diseases Cell Line Tumor medicine Biomarkers Tumor Humans Genes Tumor Suppressor RNA Messenger neoplasms Aged Retrospective Studies Aged 80 and over Analysis of Variance B-Lymphocytes Oncogene Reverse Transcriptase Polymerase Chain Reaction Large-cell lymphoma General Medicine Cell cycle Middle Aged Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease Prognosis Lymphoma Acid Anhydride Hydrolases Neoplasm Proteins Survival Rate Oncology Multivariate Analysis Cancer research Immunohistochemistry Female Diffuse large B-cell lymphoma |
| Zdroj: | Oncology reports. 11(2) |
| ISSN: | 1021-335X |
| Popis: | This study was conducted to evaluate the expression of the presumptive tumor suppressor gene, FHIT (fragile histidine triad) and its clinical significance in non-Hodgkin's lymphoma (NHL). Nine lymphoma cell lines, 62 NHL samples including 31 diffuse large B-cell lymphomas (DLBCLs), and 10 benign hyperplastic lymph nodes were analyzed for FHIT transcription using RT-PCR, with DNA sequencing performed where aberrant transcripts were found. Immunohistochemistry (IHC) studies were conducted for evaluation of FHIT protein expression with clinical data collected for assessment of the clinical relevance of the FHIT expression in DLBCLs. Eight of nine (88.9%) cell lines showed abnormal FHIT mRNA transcription, including 4 with co-existence of normal and aberrant transcripts, and 4 with absence of mRNA expression. Abnormal FHIT mRNA expression was noted in 10 (32.3%) DLBCL patients, including 3 with loss of FHIT mRNA expression and 7 with aberrant transcripts. In all NHLs, abnormal FHIT transcripts were determined for 17 of 62 patients (27.4%). Regarding the FHIT protein expression in DLBCLs, 41.9% showed strong FHIT IHC expression (2+), where 58.1% patients revealed reduced (1+) or absence (0) of FHIT protein. Decreased FHIT protein expression was associated with a worse survival after univariate and multivariate analysis. Our result indicates that abnormal FHIT mRNA expression is noted frequently in NHL cell lines and a certain proportion of NHL patients, and decreased FHIT protein expression indicates a significantly worse prognosis in DLBCLs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|