Characterization of effector functions of v-raf/mil- and v-myc-transformed murine splenic macrophage cell lines
| Autor: | William S. Walker, Andrea M. Harris, Jeffrey D. Hasday, Elizabeth M. Shah, Suzanne H. Giannini, Susan M. Roberson |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Phagocytosis
Immunology Retroviridae Proteins Oncogenic DNA Recombinant Dose-Response Relationship Immunologic Gene Expression Spleen Biology law.invention Cell Line Oncogene Proteins v-raf Mice law mental disorders Gene expression medicine Macrophage Animals Leishmania major Peritoneal Cavity Immunity Cellular Mice Inbred C3H Tumor Necrosis Factor-alpha Macrophages Antibody-Dependent Cell Cytotoxicity Oncogenes Macrophage Activation biology.organism_classification Blotting Northern Cell Transformation Viral Cell biology medicine.anatomical_structure Cell culture Leishmania tropica Recombinant DNA Tumor necrosis factor alpha human activities |
| Zdroj: | Cellular immunology. 136(2) |
| ISSN: | 0008-8749 |
| Popis: | We have characterized several of the cytocidal effector functions of a series of cell lines derived by recombinant retroviral transformation of individual clones of C3H/HeJ mouse splenic macrophages. The three cell lines described in this report (4.01, 4.07, 4.14) all expressed equivalent tumoricidal activity against P815 tumor target cells. However they differed in their high avidity binding of tumor cells (4.01 = 4.14 greater than 4.07), as well as in the killing of Leishmania major (4.01 = 4.07 greater than 4.14), the expression of antibody-dependent cellular cytotoxicity against chicken erythrocytes (4.14 greater than 4.01 greater than 4.07), and finally, in the tumor-stimulated release of tumor necrosis factor-alpha (4.01 = 4.14 greater than 4.07). The stable and restricted expression of distinct effector functions among these three cell lines makes them particularly valuable as models for establishing the precise mechanisms by which cytocidal functions are effected. In addition, they should also prove of value in understanding the basis for macrophage functional diversity. |
| Databáze: | OpenAIRE |
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