Local NO synthase inhibition produces histological and functional recovery in Achilles tendon of rats after tenotomy

Autor: Karen Renata Matos Oliveira, Anderson Manoel Herculano, Suellen Alessandra Soares de Moraes, D.L.W. Picanço-Diniz, Maria Elena Crespo-Lopez
Rok vydání: 2013
Předmět:
Zdroj: Cell and Tissue Research. 353:457-463
ISSN: 1432-0878
0302-766X
DOI: 10.1007/s00441-013-1662-2
Popis: Repair of injured tendon is a very slow process and involves the release of many molecules, including nitric oxide. We investigate the influence of local nitrergic inhibition in histological and functional recovery of injured Achilles tendon. A standard murine model of tendon injury by rupture was used. The animals were divided into three experimental groups: control, injury + vehicle (normal saline) and injury + Nω-nitro-L-arginine methyl ester (L-NAME). The products were injected into the paratendinous region every 2 days and body weight gain and Achilles functional index (AFI) were evaluated on days 0, 7, 14 and 21 after tendon injury. On day 21 post-injury, the animals were killed to evaluate nitric oxide production and tissue organization. We observed that tendon surgical division led to increased tissue nitrite levels, which were reduced in L-NAME-treated rats. The AFI revealed functional recovery of L-NAME-treated animals on day 21 post-injury, which was not observed in the saline-treated group. Microscopic analysis of hematoxylin-eosin staining and collagen autofluorescence showed that L-NAME-treated rats had more aligned areas of collagen fibers and that the diameter of newly organized collagen in this group was also greater than that in the vehicle-treated one. We demonstrate that local treatment with L-NAME significantly improves the functional parameters and accelerates histomorphological recovery.
Databáze: OpenAIRE
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