Human immunodeficiency virus in plasma and genital secretions during the menstrual cycle
| Autor: | Claudia Goulston, James I. Mullins, Eva Stevens, Carl V. Hanson, Dana Gallo, David Katzenstein |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Immunoglobulin A
Adult HIV Antibodies Virus Acquired immunodeficiency syndrome (AIDS) Proviruses medicine Immunology and Allergy Humans Viral shedding Menstrual Cycle biology Transmission (medicine) virus diseases RNA HIV Genitalia Female Middle Aged medicine.disease Virology Infectious Diseases medicine.anatomical_structure Immunology DNA Viral biology.protein Vagina RNA Viral Female Antibody |
| Zdroj: | The Journal of infectious diseases. 174(4) |
| ISSN: | 0022-1899 |
| Popis: | Six human immunodeficiency virus (HIV)-positive women were studied weekly over 8 weeks to detect HIV RNA in plasma and cervical secretions and proviral DNA in cervical, vaginal, and cervicovaginal lavage samples by polymerase chain reaction (PCR) amplification techniques. In cervical swab samples, cell-free HIV RNA was detected more frequently than cell-associated HIV proviral DNA (22/48 vs. 7/48, respectively). Cervical HIV RNA was consistently detected in 2 women with plasma HIV RNA>100,000 copies/mL but was not detected in 2 women with plasma HIV RNA < 10,000 copies/mL, regardless of menstruation status. HIV -specific IgA was detected in the plasma of 2 women and in at least 1 cervicovaginal lavage sample from all 6 women. Thus, quantitation of cervical HIV RNA can be accomplished by PCR techniques and may be useful in evaluating genital viral shedding. Transmission of human immunodeficiency virus (HIV) pro vides evidence that virus is present in genital secretions of women. HIV has been isolated from cells and cell-free fluids from female genital secretions [1-3] and cell-associated HIV proviral DNA has been detected by polymerase chain reaction (Pf.R) amplification ofcells from the genital tract in association with oral contraceptive use, cervical mucopus, cervical ectopy, pregnancy, and inflammation [4, 5]. However, genital tract shedding ofcell-free HIV RNA in virions has not been reported in HIV-infected women. Whether hormonal cycling and men struation or local immune responses, including HIV-specific IgA antibodies in genital secretions [6], affect shedding ofHIV RNA in genital secretions has not been defined. Six HIV seropositive women were followed weekly over 8 weeks to detect HIV RNA in plasma and cervical secretions, HIV provi ral DNA in cells from cervical and vaginal swabs and cervico vaginal lavage samples, and HIV-specific IgA antibodies in plasma and cervicovaginal lavage samples. |
| Databáze: | OpenAIRE |
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