Thymosin β4 reduces IL-17-producing cells and IL-17 expression, and protects lungs from damage in bleomycin-treated mice
| Autor: | Enrico Conte, Elisa Gili, Salvatore Cuzzocrea, Maria Iemmolo, Emanuela Esposito, Evelina Fagone, Mary Fruciano, Tiziana Genovese, Carlo Vancheri |
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| Rok vydání: | 2014 |
| Předmět: |
Thymosin beta 4
IL-17 Lung Inflammation Fibrosis Mice IPF Male Pathology medicine.medical_specialty Pulmonary Fibrosis Immunology Anti-Inflammatory Agents Inflammation Peptide Bleomycin Mice Random Allocation chemistry.chemical_compound Fibrosis Leukocytes medicine Animals Immunology and Allergy Lung chemistry.chemical_classification Antibiotics Antineoplastic business.industry Interleukin-17 Histology Lung Injury Hematology respiratory system medicine.disease respiratory tract diseases Thymosin Disease Models Animal medicine.anatomical_structure chemistry Immunohistochemistry Collagen Interleukin 17 medicine.symptom business Bronchoalveolar Lavage Fluid |
| Zdroj: | Immunobiology. 219:425-431 |
| ISSN: | 0171-2985 |
| DOI: | 10.1016/j.imbio.2014.02.001 |
| Popis: | Thymosin β4 (Tβ4) is a highly conserved peptide with immunomodulatory properties. In this research we investigated the effects of Tβ4 on the bleomycin-induced lung damage in CD-1 mice and the changes in the number of IL-17-producing cells as well as the IL-17 expression in the lung. Male CD-1 mice were treated with bleomycin (1mg/kg) in the absence or the presence of Tβ4 (6mg/kg delivered intra-peritoneally on the day of bleomycin treatment and for 2 additional doses). After sacrifice one week later, lung histology, measurement of collagen content of the lung, Broncho Alveolar Lavage Fluid (BALF) analysis, evaluation of IL17-producing cells in the blood as well as RT-PCR and IHC in the lung tissue were performed. As expected, bleomycin-induced inflammation and lung damage were substantially reduced by Tβ4 treatment in CD-1 mice, as shown by the significant reduction of (i) leukocytes in BALF, (ii) histological evidence of the lung damage, and (iii) total collagen content in the lung. Importantly, the bleomycin-induced increase in the number of IL17-producing cells in the blood was significantly blocked by Tβ4. Accordingly, IHC and RT-PCR results demonstrated that Tβ4 substantially inhibited bleomycin-induced IL-17 over-expression in the lung tissue. This is the first report showing that a decreased amount of IL17-producing cells and inhibited IL-17 expression in the lung with Tβ4 treatment correlate with its anti-inflammatory and anti-fibrotic effects. |
| Databáze: | OpenAIRE |
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