Enhancement of Cell Adhesion, Cell Growth, Wound Healing, and Oxidative Protection by Gelatins Extracted from Extrusion-Pretreated Tilapia (Oreochromis sp.) Fish Scale

Autor:	Shu-Ling Hsieh, Ren-Han Huang, Hui-Ru Guo, Yong-Han Hong, Tien-Chou Wu, Chun-Yung Huang
Rok vydání:	2018
Předmět:	0301 basic medicine oxidative protection food.ingredient tilapia human keratinocyte-derived cell line HaCaT Pharmaceutical Science wound healing Gelatin Analytical Chemistry gelatin lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology food lcsh:Organic chemistry Drug Discovery cell growth Food science Physical and Theoretical Chemistry Cell adhesion Akt/mTOR pathway PI3K/AKT/mTOR pathway integumentary system Chemistry Cell growth Organic Chemistry cell adhesion 04 agricultural and veterinary sciences 040401 food science Hot water extraction HaCaT extrusion 030104 developmental biology Chemistry (miscellaneous) Molecular Medicine Citric acid Wound healing
Zdroj:	Molecules, Vol 23, Iss 10, p 2406 (2018) Molecules Volume 23 Issue 10
ISSN:	1420-3049
DOI:	10.3390/molecules23102406
Popis:	Gelatin has been broadly utilized in the food, pharmaceutical, photographic, cosmetic and packaging industries, and there is also huge potential for novel applications of gelatin in the fields of biotechnology and biomedicine. In the present study, we extracted gelatin from fish processing waste, i.e., scale of tilapia, by a combined method of extrusion-pretreatment and hot water extraction. The extrusion-pretreatment process increases the extraction yield of gelatin. Three gelatins (FS2: preconditioning with double-distilled water (ddH2O) before extrusion FS12: preconditioning with citric acid solution before extrusion FS14: preconditioning with acetic acid solution before extrusion) were obtained and all of them enhanced cell adhesion, cell growth, and wound healing in HaCaT cells and protected HaCaT cells from H2O2-induced cellular damage. Among FS2, FS12, and FS14, FS12 exhibited the most pronounced enhancement of cell adhesion, cell growth, and wound healing in HaCaT cells, and thus it may have potential as an effective natural raw material in cell therapies for cutaneous wounds and for reducing H2O2-induced oxidative damage of cells. In additional experiments, it was found that phosphorylations of Akt and mTOR are involved in the signaling pathway activated by FS2, FS12, and FS14 in HaCaT cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2dfb31bc9d02eb0ff95ef6e9d7e555f6 https://doi.org/10.3390/molecules23102406 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.