Immunolocalisation of E-cadherin and -catenin in human pterygium
| Autor: | Izuru Sato, Kazuhiko Yoshida, Mitsuhiko Osaki, Katsuya Nakanishi, Shuji Takahashi, Hisao Ito, Shigeaki Ohno, Satoru Kase |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Pathology medicine.medical_specialty Conjunctiva Stromal cell Scientific Report Biology Pterygium Immunoenzyme Techniques Cellular and Molecular Neuroscience Cornea Cell Adhesion medicine Humans Eye Proteins Cell adhesion beta Catenin Aged Aged 80 and over Pterygium (conjunctiva) Cadherin Cell adhesion molecule Epithelium Corneal Epithelial Cells Middle Aged Cadherins medicine.disease eye diseases Sensory Systems Epithelium Ophthalmology medicine.anatomical_structure Female sense organs |
| Zdroj: | British Journal of Ophthalmology. 91:1209-1212 |
| ISSN: | 0007-1161 |
| DOI: | 10.1136/bjo.2007.115709 |
| Popis: | Aim: The pterygium represents an invasion of a wing of altered ocular surface tissue into the normal cornea. The head itself is slightly elevated and white, which is the only site of firm adhesion to the globe. The mechanisms of cell proliferation and adhesion in pterygium epithelium, however, are unknown. The aim of this study was to analyse the expression of cell adhesion molecules in pterygium tissues. Methods: Six pterygia were surgically removed using the bare-sclera procedure, and two normal corneas and a normal bulbar conjunctiva were also obtained. Formalin-fixed, paraffin-embedded tissues were analysed by immunohistochemistry with anti-E-cadherin and β-catenin antibodies. Results: Immunoreactivity for E-cadherin was not detected in the normal cornea and conjunctiva. In contrast, all corneal and conjunctival epithelial cells showed a weak homogeneous immunoreaction for β-catenin on the cell membrane. In the pterygium head, the thickness was relatively marked compared with the body, and normal conjunctival and corneal epithelia. E-cadherin as well as β-catenin was heterogeneously expressed in the cell membrane and cytoplasm of a variety of epithelial cells, whereas the expression was less marked in the body. Several epithelial cells showed intense nuclear immunoreactivity for β-catenin. Immunoreactivity of β-catenin, but not E-cadherin, was detected in only a few stromal cells, which were less marked than in epithelial cells. Conclusion: It is suggested that E-cadherin and β-catenin are concentrated in pterygium tissue, and are possibly involved with epithelial proliferation and adhesion. |
| Databáze: | OpenAIRE |
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